Vaidlustaja täiendused ja taotlused

18.08.2025 VAIDLUSTUSE ALUSE TÄIENDAMINE JA TAOTLUS TEABE VÄLJASTAMISEKS JA TAOTLUS UUTE TÄHTPÄEVADE MÄÄRAMISEKS Riigihange: „Digipatoloogia (WSI) skanneri ostmine“ viitenumber: 292949 Vaidlustaja: Osaühing Balti Intermed registrikood: 10170298 Esindaja: Erki Fels, vandeadvokaat Gregor Saluveer, vandeadvokaadi abi Advokaadibüroo TEGOS e-post: [email protected] Hankija: Aktsiaselts Lääne-Tallinna Keskhaigla registrikood: 10822269 Esindaja: Kaisa Suvi, jurist e-post: [email protected] Kolmas isik: Quantum Eesti AS registrikood: 10832463 e-post: [email protected] Esindaja: Aarne Akerberg, vandeadvokaat Advokaadibüroo Lillo & Partnerid e-post: [email protected] TAOTLUSED 1. Võtta vastu vaidlustuse aluse täiendamine nõude raames, mis puudutab Quantum Eesti AS pakkumuse mittevastavust. 2. Kohustada Aktsiaseltsi Lääne-Tallinna Keskhaigla väljastama Osaühingule Balti Intermed: 2.1. Quantum Eesti AS pakkumuse dokument „1_2_3_Pakutav komplektsus“; 2.2. Quantum Eesti AS pakkumuse dokument, milles sisaldub pakutava seadme Aperio GT 450 kasutusjuhend; 2.3. Aktsiaseltsi Lääne-Tallinna Keskhaigla ja Quantum Eesti AS teabevahetus kuupäevadest 18.06.2025 ja 25.06.2025; Advokaadibüroo TEGOS AS Liivalaia 36, 10132 Tallinn Kaluri 2, 51004 Tartu [email protected] Telefon +372 626 4300 Telefon +372 730 1610  2.4. Quantum Eesti AS 25.06.2025 sõnumile lisatud manused, sealhulgas dokument „GT450&Cytolog_vs1.pdf“. 3. Määrata vaidlustusmenetluses uued tähtpäevad. 1. ASJAOLUD 1.1. Riigihangete vaidlustuskomisjoni (VAKO) menetluses on Osaühingu Balti Intermed (vaidlustaja) vaidlustus Aktsiaseltsi Lääne-Tallinna Keskhaigla (hankija) riigihankes „Digipatoloogia (WSI) skanneri ostmine“ (vr 292949). Menetlusse on kolmanda isikuna kaasatud Quantum Eesti AS (kolmas isik). 1.2. 15.08.2025 esitasid hankija ja kolmas isik vastuse vaidlustusele. 1.3. VAKO määras täiendavate seisukohtade esitamise tähtpäevaks 21.08.2025. 1.4. Vaidlustaja täiendab käesolevaga vaidlustuse alust (RHS § 191 lg 1), kuna kolmanda isiku vastusest vaidlustusele on ilmnenud täiendav pakkumuse mittevastavus (RHS § 114 lg 2). 2. PÕHJENDUSED 2.1. Vaidlustaja selgitas vaidlustuses, et kolmanda isiku pakkumus on mittevastav järgmisele osale TK p-st 1: „Seade peab võimaldama nii histoloogiliste kui tsütoloogiliste preparaatide skaneerimist.“ 2.2. Vaidlustaja selgitas, et talle teadaolevalt pakkus kolmas isik toodet Aperio GT 450 koos DX ja RUO tarkvaraga. Vaidlustaja esitas selle toote kasutusjuhendi DX tarkvaraga, milles oli kirjas, et seade pole ette nähtud tsütoloogiliste preparaatide skaneerimiseks. Seega oli mittevastavus ilmne. 2.3. Vaidlustuse vastuses selgitab kolmas isik, et ta ei paku Aperio GT 450 skannerit DX tarkvaraga, vaid Z-Stack lisamooduliga tsütoloogiliste preparaatide skaneerimiseks.1 2.4. Kolmanda isiku vastusest järeldub mittevastavus järgmisega osaga TK p-st 1: „Kui pakutav seade on kasutatav nii IVD-le vastava tarkvaraga (nõutud), kui ka RUO tarkvaraga (valikuline), peab pakkumus sisaldama võimalust paigaldada hankijale sobiv tarkvara versioon ilma lisakuluta.“ 2.5. Sellest järeldub, et nõutud on pakkuda seadet IVD-le vastava ehk in vitro diagnostic tarkvaraga, RUO tarkvaraga seadme pakkumine on valikuline. IVD tähendab seda, et seadet saab kasutada diagnostikas ehk kliinilises töös. 2.6. Kolmanda isiku pakutud skanner on IVD sertifitseeritud üksnes DX (vt vaidustuse lisa 1). Kui kolmas isik pole pakkunud Aperio GT 450 skannerit DX tarkvaraga, nagu ta väidab, järeldub sellest, et ta pakub vaid RUO tarkvara. RUO tarkvara on kasutamiseks ainult teadustöös, mitte diagnostikas. 2.7. Vaidlustaja leidis avalikest allikatest Aperio GT 450 kasutusjuhendi, mille on kolmas isik pakkumuses esitanud (lisa 1). Selle lk-l 10 on kirjutatud: 1 Kolmanda isiku vastus vaidlustusele, p-d 6.1 ja 6.2. 2/4 2.8. Vaidlustaja on avalikest allikatest leidnud ka artikli selle kohta, et tootja on lisanud Aperio GT 450 skannerile Z-stack funktsiooni. Samas on märge, et tarkvara on kasutatav üksnes teadustöös, mitte diagnostikas (lisa 2): 2.9. Kokkuvõttes on kolmanda isiku pakkumus mittevastav ka ülalviidatud osale TK p-st 1, mis nõuab seadme kasutatavust IVD-le vastava ehk diagnostikas kasutatava tarkvaraga. 2.10. Kolmanda isiku pakkumus on jätkuvalt mittevastav ka sellele osale TK p-st 1, mis sätestab, et seade peab võimaldama tsütoloogiliste preparaatide skaneerimist. „Seade“ tähendab selles lauses IVD sertifitseeritud tarkvaraga seadet, kuna just sellise seadme pakkumine oli kohustuslik. Kuna kolmas isik ei ole pakkunud IVD sertifitseeritud tarkvaraga seadet, ei sisalda ta pakkumus „seadet“, mis võimaldab tsütoloogiliste preparaatide skaneerimist diagnostika eesmärgil. 3. MENETLUSLIKUD KÜSIMUSED 3.1. Taotlus teabe väljastamiseks. Vaidlustaja palub talle väljastada kolmanda isiku pakkumuse komplektsuse tabeli, kasutusjuhendi, teabevahetuse ja teabevahetuse manused. Kõik need dokumendid puudutavad küsimust sellest, kas kolmanda isiku pakutud seadmel on nõutud funktsionaalsused või mitte: a. Komplektsuse tabel ei ole selgelt ärisaladus, kuna kätkeb teavet, millist toodet on kolmas isik pakkunud. Samasuguse tabeli on koostanud vaidlustaja ise ja hankija ei ole pidanud kohatuks selle tabeli sisu vaidlustusmenetluses lahata. b. Ärisaladuseks ei ole ka pakkumuses esitatud kasutusjuhend, kuna sellel puudub kaubanduslik väärtus (tootja ise teeb kasutusjuhendid avalikult kättesaadavaks). Kasutusjuhend on vaja vaidlustajale väljastada selleks, et kontrollida, kas avalikest allikatest leitud kasutusjuhend on sama, mille on kolmas isik esitanud pakkumuses. Kui mitte, soovib vaidlustaja kontrollida, kas seadme tarkvara on IVD sertifitseeritud või mitte. c. Eelnevaga samal põhjusel ei ole ärisaladuseks teabevahetus, kuna käsitleb samamoodi üksnes teavet pakutud seadme funktsionaalsuste kohta (mis peaksid niigi kasutusjuhendist nähtuma). 3.2. Taotlus uute tähtpäevade määramiseks. Arvestades vaidlustuse aluse täiendamist palub vaidlustaja VAKO-l määrata vaidlustusmenetluses uued tähtpäevad. VAKO varasem praktika vaidlustuse aluse täiendamise korral on sama (lisa 3). Tähtpäevade muutmisega ei satu ohtu VAKO otsuse tähtaegne tegemine, kuna vaidlustuse aluse täiendamine annab 3/4  otsuse tegemiseks 5 päeva juurde (RHS § 200 lg 1). Praegu on määratud vaidlustajale tähtpäev seisukoha esitamiseks 21.08.2025 ning hankijale ja kolmandale isikule 26.08.2025. See tähendab, et uue vaidlustuse aluse osas ei ole vaidlustajal enam võimalik hankija ega kolmanda isiku seisukohtadele vastata. Vaidlustaja palub määrata tähtajad: 1) hankijale ja kolmandale isikule vastuse esitamiseks ainult vaidlustuse aluse täiendamisele; 2) vaidlustajale täiendavate seisukohtade esitamiseks kogu vaidlustuse osas; ja 3) hankijale ja kolmandale isikule vastamiseks vaidlustaja täiendavale seisukohale. 3.3. Tähtaegsus. VAKO määras täiendavate seisukohtade esitamise tähtpäevaks 21.08.2025. Vaidlustuse aluse täiendamine on tähtaegne (RHS § 191 lg 1). 3.4. Esitamine. Dokument on esitatud VAKO-le ([email protected]) ja menetlusosalistele e-kirja teel. Lugupidamisega (digitaalselt allkirjastatud) Erki Fels, Gregor Saluveer, vandeadvokaat vandeadvokaadi abi LISAD Lisa 1 Aperio GT 450 kasutusjuhend Lisa 2 Artikkel Lisa 3 VAKO varasem praktika tähtaegade osas 4/4  L E I C A B I O S Y S T E M S I M A G I N G APERIO GT 450 DIGITAL PATHOLOGY SLIDE SCANNER USER'S GUIDE (NOT for use in China) Aperio GT 450 User's Guide MAN-0391, Revision G | December 2024 This manual applies to Aperio GT 450 Controller, Aperio GT 450 Console, and Aperio GT 450 SAM versions 1.4 and later. Original Instructions. Copyright Notice l Copyright © 2019 - 2024 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are trademarks and registered trademarks of Leica Microsystems IR GmbH. Aperio, Aperio iQC, GT, and GT 450 are trademarks of Leica Biosystems Imaging, Inc. in the USA and optionally in other countries. Other logos, products, and/or company names might be trademarks of their respective owners. l This product is protected by registered patents. For a list of patents, contact Leica Biosystems. Customer Resources l For the latest information on Leica Biosystems Aperio products and services, please visit LeicaBiosystems.com/Aperio. Contact Information – Leica Biosystems Imaging, Inc. Headquarters Customer Support Leica Biosystems Imaging, Inc. Contact your local support representative with any query or service request. 1360 Park Center Drive Vista, CA 92081 https://www.leicabiosystems.com/contact-us/ USA Tel: +1 (866) 478-4111 (toll free) Direct International Tel: +1 (760) 539-1100 Importers Leica Biosystems Deutschland GmbH Leica Microsystems (UK) Limited Heidelberger Straße 17-19 Larch House, Woodlands Business Park 69226 Nussloch, Germany Milton Keynes, England, United Kingdom, MK14 6FG For research use only. Not for use in diagnostic procedures. 00815477020228, 00815477020464, 00815477020471, 00815477020563, 00815477020495 23GT450, 23GT450SAM, 23GT450SAMSW, 23GT450ZSTACKSW, 23GT450-R Aperio GT 450 User's Guide, MAN-0391 Revision G 2 Copyright © 2024 Leica Biosystems Imaging, Inc. Table of contents Notices 8 Revision Record 8 Cautions and Notes 9 Intended Use 10 Symbols 11 1 Introduction 14 Resources 16 Warnings 16 Electromagnetic warnings 16 Instrument warnings 17 Component and part replacement warnings 17 Aperio GT 450 Compliance Specifications 17 Installation 18 Disposal of the Aperio GT 450 19 Scanner safety instructions 19 2 Aperio GT 450 overview 22 Theory of operation 23 Scanner overview 23 Turn the scanner on and off 24 Touchscreen interface overview 25 Home screen 25 Help videos and guides 27 Key features 28 Continuous load workflow 28 Scan magnification 28 Automatic image quality check 28 Manual Scanning 28 Z-Stack scanning 28 Extended Focus 28 Image types supported 28 Slide types supported 28 Coverslips 28 Aperio GT 450 User's Guide, MAN-0391 Revision G 3 Copyright © 2024 Leica Biosystems Imaging, Inc.  Slide racks supported 29 Slide capacity 29 Barcodes supported 29 Optional Aperio Viewing Station 29 Aperio Digital Slide Viewers 30 3 Slide preparation 31 Slide preparation overview 32 Slide preparation checklist 33 Tissue preparation 33 Staining 33 Slide preparation guidelines 34 Tissue placement 34 Fixing slide preparation errors 34 Coverslips 35 Labels 35 Barcodes 36 Slide quality control 36 General guidelines for slide preparation 37 4 Scan slides 40 Scanning concepts 41 Scan workflow 41 Carousel rotation 42 Log in and log out of the scanner 42 Load slides in the rack 43 Load racks in the carousel 44 Priority scanning 46 20x magnification scanning 47 Change the scan magnification for a rack of slides 47 Z-Stack scanning 49 Scan a rack of slides using z-stack scanning 49 View z-stack images 51 Extended Focus scanning 52 Scan a rack of slides using Extended Focus 52 Aperio GT 450 User's Guide, MAN-0391 Revision G 4 Copyright © 2024 Leica Biosystems Imaging, Inc.  Rotate a rack to the rack loading area 55 Unload racks from the carousel 56 Check scan status 57 Check rack status 58 Rack error on current scan 58 View slide status for a rack 59 View macro images of scanned slides 60 View rack scan order 60 Rack warnings 61 Scan entire slide for whole rack 63 Image quality control for histotechnicians and pathologists 64 Check that the scanned image covers all slide tissue 64 Scan statistics 66 Manual Scanning 67 Manual Scan overview 67 Manual Scan user interface 67 Use Manual Scan to resolve an image quality issue 67 Aperio iQC Software Module Notifications 72 5 Maintenance 73 Maintenance schedule 74 Serial number and firmware version 74 Shut down the scanner 75 Open the scanner cover and access the interior components 75 Recommended daily maintenance 77 Restart the scanner 78 Inspect the stage slide tray 79 Inspect the pusher/puller 81 Six month maintenance 82 Clean the objective and Koehler 82 Materials needed 83 Clean the stage slide tray 85 Clean the carousel 87 Clean the fan filter 88 Clean the slide racks 90 Aperio GT 450 User's Guide, MAN-0391 Revision G 5 Copyright © 2024 Leica Biosystems Imaging, Inc.  Clean the scanner cover 90 Clean the touchscreen 91 Restart the scanner after interior maintenance 91 Transporting or moving the Aperio GT 450 92 Long term storage 92 Scanner Maintenance Log 93 6 Troubleshooting 94 Personal protective equipment 95 Red blinking lights on carousel 95 How to use the troubleshooting steps 95 Perform a safe restart after an error 96 Error codes and solutions 99 1000: Internal error 99 1001: Scanner cannot initialize 103 1002: Carousel cannot rotate 106 1003: Carousel cannot rotate. Carousel pinch point obstructed. 108 1007: Internal storage full. Cannot send images to DICOM converter. 108 2000: Slide handling error at slide stage, rack, or pusher. 109 2001: Slide handling error at rack gripper, lift, or carousel. 113 9000: Scanner cover is open 115 Rack warnings and solutions 115 1005: Cannot process rack 116 1006: Cannot process one or more slides in rack 116 Slide errors and solutions 117 Tilted slide(s) 117 No barcode 118 No tissue 118 No macro focus 119 Image quality 119 Image transfer error – pending retry 120 Aborted 121 Aborted message appears on all slides 121 Artifact detection messages from the optional Aperio iQC Software Module 123 Symptoms and solutions 123 Aperio GT 450 User's Guide, MAN-0391 Revision G 6 Copyright © 2024 Leica Biosystems Imaging, Inc.  Scanner does not power on 123 Touchscreen does not respond to touch 124 Touchscreen is black 124 Slides are broken inside the scanner 124 Network connection lost 124 White striping appears on scanned images 126 Index 127 Aperio GT 450 User's Guide, MAN-0391 Revision G 7 Copyright © 2024 Leica Biosystems Imaging, Inc. Notices Important message Service personnel and distributors who have access to protected patients' information must treat all such information as confidential in accordance with professional ethics, accreditation standards, and legal requirements. Revision Record Rev. Issued Sections Affected Detail G December Chapter 3: Slide Chapter 3: Slide preparation: Added instructions to clarify slide preparation 2024 preparation requirements: Slide preparation checklist (on page 33); Slide quality control (on page 36); General guidelines for slide preparation (on page 37) Chapter 4: Scan slides Chapter 4: Scan slides Added instructions for using new features: Manual Scanning (on page 67); Extended Focus scanning (on page 52); Aperio iQC Chapter 5: Software Module Notifications (on page 72) Maintenance Chapter 5: Maintenance: Added the following daily maintenance Chapter 6: recommendations: Inspect the stage slide tray (on page 79); Inspect the Troubleshooting pusher/puller (on page 81). Added a caution statement to clarify instructions: Restart the scanner after interior maintenance (on page 91). Clarified instructions for Clean the objective and Koehler (on page 82). Added Scanner Maintenance Log (on page 93) Chapter 6: Troubleshooting: Revised troubleshooting procedures to improve clarity or include additional instructions. In Slide errors and solutions (on page 117) section, revised No barcode (on page 118); No tissue (on page 118); Image quality (on page 119)Image quality (on page 119) Added White striping appears on scanned images (on page 126) F October Copyright page, Added UKCA symbol and CE mark on on page 2, and the UKCA symbol to 2024 "Notices;" Chapter the Symbols (on page 11); Revised Symbols on page 11 to meet current 1, "Introduction;" compliance requirements; Chapter 1: Revised Aperio GT 450 Compliance Chapter 2, "Aperio Specifications (on page 17) to meet current compliance requirements; GT 450 overview;" Chapter 2: Added Theory of operation (on page 23); revised Key features Chapter 4, "Scan (on page 28) to include Release 1.3 features; Chapter 4: Added 20x slides;" Chapter 6, magnification scanning (on page 47); added Z-Stack scanning (on page 49); "Troubleshooting" added Check that the scanned image covers all slide tissue (on page 64); Chapter 6: Revised 1007: Internal storage full. Cannot send images to DICOM converter. (on page 108) for clarity; revised Image transfer error – pending retry (on page 120) for clarity; revised Network connection lost (on page 124) for clarity. Aperio GT 450 User's Guide, MAN-0391 Revision G 8 Copyright © 2024 Leica Biosystems Imaging, Inc.  Rev. Issued Sections Affected Detail E March 2022 Front matter, Added revision history, cautions and notes, updated compliance Compliance specifications; Chapter 4: Updated scan workflow diagram to include Specifications, manual image quality check. Added new section “Image Quality Control for Chapter 4, "Scan Histotechnicians and Pathologists;" Chapter 6: Added troubleshooting Slides," Chapter 6, section for lost Internet connection. “Troubleshooting” D April 2020 All Changed references to two monitors to “monitor(s)” to accommodate change in product configuration. C November All Updated graphics for patch 1.0.1. 2019 B October All Changes for patch 1.0.1. Updated graphics, added new section “Scan Entire 2019 Slide for Whole Rack.” A June 2019 All New document. Cautions and Notes l Serious Incidents Reporting — Any serious incident that has occurred in relation to the Aperio GT 450 shall be reported to the manufacturer and the competent authority of the member state in which the user is established. l Specifications and Performance — For device specifications and performance characteristics, see the document Aperio GT 450 Specifications. l Installation — Aperio GT 450 must be installed by a trained Leica Biosystems Technical Services representative. l Repair — Repairs may be done only by a trained Leica Biosystems Technical Services representative. After repairs are done, ask the Leica Biosystems technician to perform operation checks to determine the product is in good operating condition. l Accessories — For information on using Aperio GT 450 with third-party accessories such as a Laboratory Information System (LIS) not provided by Leica Biosystems, contact your Leica Biosystems Technical Services representative. l Quality Control — For information on image quality checks, see Image quality control for histotechnicians and pathologists (on page 64). l Maintenance and Troubleshooting — For information on maintenance and troubleshooting, see the Aperio GT 450 User’s Guide. Aperio GT 450 User's Guide, MAN-0391 Revision G 9 Copyright © 2024 Leica Biosystems Imaging, Inc.  l Cybersecurity — Be aware that workstations are susceptible to malware, viruses, data corruption, and privacy breaches. Work with your IT administrators to protect workstations by following your institution’s password and security policies. To protect workstations and servers from malware intrusion, use caution when inserting USB drives and other removable devices. Consider disabling USB ports that are not in use. If you plug in a USB drive or other removable device, you should scan the devices with an anti-malware utility. For Aperio recommendations on protecting your workstations and servers, see the Aperio GT 450 IT Manager and Lab Administrator Guide. If a suspected Aperio GT 450 cybersecurity vulnerability or incident is detected, contact Leica Biosystems Technical Services for assistance. As a system security measure, Leica Biosystems products capture and log external attempts to access system data. For more information, contact your Leica Biosystems representative. l Training — This manual is not a substitute for the detailed operator training provided by Leica Biosystems or for other advanced instruction. l Safety — This device is intended for indoor use only. Safety protection may be impaired if this device is used in a manner not specified by the manufacturer. Intended Use For research use only. Not for use in diagnostic procedures. Aperio GT 450 User's Guide, MAN-0391 Revision G 10 Copyright © 2024 Leica Biosystems Imaging, Inc. Symbols The following symbols appear on your product label or in this user guide: Symbol Regulation/Standard Description ISO 15223-1 - 5.4.3 Consult Instructions for Use ISO 15223-1 - 5.1.1 Manufacturer ISO 15223-1 - 5.1.3 Date of manufacture ISO 15223-1 - 5.1.8 Importer 21 CFR 809.10(c)(2)(i) For research use only. Not for use in diagnostic procedures. ISO 15223-1 - 5.1.7 Serial number ISO 15223-1 – 5.1.6 Catalog number ISO 15223-1 – 5.7.10 Unique Device Identifier Machinery Directive 2006/42/EC Device carries the CE (Conformitè Europëenne) Mark and fulfils the requirements of Machinery Directive 2006/42/EC and additional EU Directives, as shown Machinery and Materials. Electromagnetic Compatibility Device is in compliance with UK Conformity Assessment Regulations 2016 and Low requirements. Voltage Electrical Equipment Regulations 1989 ISO 15223-1 - 5.4.4 Caution SO 7010 – W001 General warning Aperio GT 450 User's Guide, MAN-0391 Revision G 11 Copyright © 2024 Leica Biosystems Imaging, Inc.  Symbol Regulation/Standard Description IEC 61010-1 TÜV Product Services have certified that the listed products comply with both U.S. and Canadian safety requirements. IEC 60417 - 5031 This device is suitable for direct current only. IEC 60417 - 5007 On. To indicate connection to the mains, at least for mains switches or their positions, and those cases where safety is involved. IEC 60417 - 5008 Off. To indicate disconnection from the mains, at least for mains switches, and all those cases where safety is involved. ISO 15523-1 5.7.3 Temperature limitation ISO 15223-1 5.3.8 Humidity limitation 2012/19/EU Device is regulated under 2012/19/EU (WEEE Directive) for Electrical and Electronic Equipment Waste and must be discarded under special conditions. People’s Republic of China Device contains certain toxic or hazardous elements and can Electronic Industry Standard be used safely during its environmental protection use SJ/T11364 period. The number in the middle of the logo indicates the environmental protection use period (in years) for the product. The outer circle indicates that this product can be recycled. National Standard of the People’s Device contains certain toxic or hazardous elements and can Republic of China Requirements be used safely during its environmental protection use of concentration limits for certain period. The "e" inside circle indicates product is compliant restricted substances in with Requirements of concentration limits for certain electrical and electronic products substances in electrical and electronic products GB/T 26572- GB/T 26572-2011 2011. The outer circle indicates that the product can be recycled. Aperio GT 450 User's Guide, MAN-0391 Revision G 12 Copyright © 2024 Leica Biosystems Imaging, Inc.  Symbol Regulation/Standard Description IEC 60825-1 Device is a Class 1 Laser Product that is in compliance with international standards and US requirements. CA Proposition 65 This product can expose you to chemicals known to the State of California to cause Cancer and Reproductive Harm. For more information go to https://www.P65Warnings.ca.gov. N/A Device is made in the USA of U.S. and foreign components. Aperio GT 450 User's Guide, MAN-0391 Revision G 13 Copyright © 2024 Leica Biosystems Imaging, Inc. 1 Introduction In this section: Resources 16 Warnings 16 Aperio GT 450 Compliance Specifications 17 Installation 18 Disposal of the Aperio GT 450 19 Scanner safety instructions 19 Aperio GT 450 User's Guide, MAN-0391 Revision G 14 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction The Aperio GT 450 is a high performance, brightfield whole slide scanner that includes continuous loading with 450-slide capacity across 15 racks, priority rack scanning, automated image quality check and a scan speed of ~32 seconds at 40x scanning magnification for a 15 mm x 15 mm area. Use of the Aperio GT 450 requires the following components. Component Description Aperio GT 450 Scanner The Aperio GT 450 SAM Client Application Software resides on a server, which is Administration Manager referred to in this document as the Aperio GT 450 SAM server. The Aperio GT 450 SAM (Aperio GT 450 SAM) Server server connects to multiple Aperio GT 450 scanners. Aperio GT 450 SAM Client The Aperio GT 450 SAM client application software enables IT implementation, PIN Application Software configuration, and service access of multiple scanners from a single desktop client location for IT professionals. Workstation, monitor, and A workstation, monitor, and keyboard must be connected to your local area network keyboard with access to the Aperio GT 450 SAM server to administer the Aperio GT 450 scanners. See the Aperio GT 450 Specifications for more information on these components. Ensure you follow appropriate good laboratory practices and the policies and procedures required by your institution for slide preparation, processing, storage, and disposal. Use this equipment only for this purpose and in the manner described in this guide. Aperio GT 450 User's Guide, MAN-0391 Revision G 15 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction Resources Resource Description Aperio GT 450 User's Guide Provides reference information and instructions for scanning, troubleshooting, and maintenance. Aperio GT 450 Quick Reference Contains quick instructions for scanning, basic troubleshooting, and maintenance. Guide We recommend keeping this printed guide with your scanner. An electronic version of the quick reference guide is available from the Help area on the scanner’s touchscreen interface. Aperio GT 450 IT Manager and Contains information and instructions regarding IT administration of the scanner, Lab Administrator's Guide including network administration and configuration. Aperio GT 450 Specifications Provides detailed specifications for the scanner. Aperio GT 450 DICOM Upgrade Provides information on setting up the optional Aperio GT 450 1.3 DICOM Feature Guide Pack. Instructional videos Provides video instruction on performing various tasks. You can view the videos from the Help area on the scanner’s touchscreen interface. Customer Support If you have an issue with the scanner you cannot resolve, contact Leica Biosystems Technical Services. Training Leica Biosystems offers classroom and virtual training courses. Contact Leica Biosystems Customer Support for information about training options. Warnings This section contains important safety and operation warnings. For more details, see also Scanner safety instructions (on page 19). Electromagnetic warnings If the scanner is used in any manner not specified in this documentation, the protection provided by the equipment may be impaired. Portable RF communications equipment (including peripherals such as antenna cables and external antennas) should be used no closer than 30 cm (12 inches) to any part of the scanner, including cables specified by the manufacturer. Otherwise, degradation of the performance of this equipment could result. This device complies with the emissions and immunity requirements as specified in the EN/1EC 61326 series of Product Family Standards for a “basic electromagnetic environment”. Such equipment is supplied directly at low voltage from public mains network. This equipment is not intended for residential use. Aperio GT 450 User's Guide, MAN-0391 Revision G 16 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction This device generates, uses, and can radiate unintentional radio-frequency (RF) energy. If this device is not installed and operated correctly, this RF energy can cause interference with other equipment. It is the responsibility of the end user to be sure that a compatible electromagnetic environment for the device can be maintained so that the device operates as intended. This equipment is designed for use in a PROFESSIONAL HEALTHCARE FACILITY ENVIRONMENT. It is likely to perform incorrectly if used in a HOME HEALTHCARE ENVIRONMENT. If it is suspected that performance is affected by electromagnetic interference, correct operation may be restored by increasing the distance between the equipment and the source of the interference. In addition, other equipment can radiate RF energy to which this device is sensitive. If one suspects interference between this device and other equipment, Leica Biosystems recommends the following actions to correct the interference: l Evaluate the electromagnetic environment before installation and operation of this device. l Do not operate this device close to sources of electromagnetic radiation (for example: unshielded intentional RF sources), as these can interfere with proper operation. Examples of unshielded intentional radiators are handheld radio transmitters, cordless phones, and cellular phones. l Do not place this device near medical electrical equipment that can be susceptible to malfunctions caused by close proximity to electromagnetic fields. l This device has been designed and tested to CISPR 11 Class A emission limits. In a domestic environment, this device can cause radio interference, in which case, you need to take measures to mitigate the interference. Instrument warnings WARNING: To reduce the risk of fire or electric shock, do not expose the scanner to rain or moisture. Misuse of electrical equipment can cause electrocution, burns, fires, and other hazards. Component and part replacement warnings There are no user-replaceable parts or components in the Aperio GT 450 scanner. Replacement of parts or components within the Aperio GT 450 scanner must be performed by qualified Leica Biosystems Technical Services personnel using Leica Biosystems specified parts. Aperio GT 450 Compliance Specifications This device complies with Part 15 of the FCC rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference and (2) this device must accept any interference received, including interference that may cause undesired operation. This device was evaluated against and complies to the following standards. Aperio GT 450 User's Guide, MAN-0391 Revision G 17 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction Feature Details Safety IEC 61010-1:2010 IEC 61010-1: 2010/AMD1:2016 IEC 61010-2-101: 2018 CAN/CSA C22.2 No. 61010-1:2012/A1:2018 CAN/CSA C22.2 No. 61010-2-101:2019 UL 61010-1:2012/R2019-07 UL 61010-2-101:2019 EN 61010-1:2010/A1:2019 EN 61010-2-101:2017 Electromagnetic EMC Directive (2014/30/EU) Compatibility (EMC) EN 61326-1:2013 CISPR 11: 2015 FCC Part 15 Subpart B ICES-003 Issue 6: 2016 CNS13438: 2006 KN 32: 2015-12 KN 35: 2015-12 Machinery and IEC 60825-1:2014 (Class 1 Laser) Materials 2011/65/EU - Restriction of the use of certain hazardous substances in electrical and electronic equipment (RoHS 2) 2015/863 - Restriction of the use of certain hazardous substances in electrical and electronic equipment (RoHS 3) 2006/42/EC - Machinery Directive 2014/35/EU – Low Voltage Directive Installation WARNING: Two people are required for lifting the scanner. Installation and setup of the scanner should only be performed by a trained Leica Biosystems Service representative. After installation, the Leica Biosystems Service representative will check the scanner for proper operation. Aperio GT 450 User's Guide, MAN-0391 Revision G 18 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction WARNING: Incorrect installation could result in serious injury to the operator or ergonomic strain. For work surface and operating specifications, see the Aperio GT 450 Specifications, MAN-0393. When you first receive the Aperio GT 450 scanner and accessories, inspect the pallet and attached boxes for any damage. If the package has visible damage from shipment, contact Leica Biosystems Customer Service for assistance. Disposal of the Aperio GT 450 The service life of the Aperio GT 450 is five years. After that time, cybersecurity patches and other technical support may not be provided. This device is regulated as Electrical and Electronic Equipment Waste (WEEE) under 2012/19/EU and must be discarded under special conditions. Contact Leica Biosystems Imaging, Inc. for assistance or questions regarding device disposal. Scanner safety instructions This section contains important safety information for the Aperio GT 450 scanner. When using your scanner, always take basic safety precautions, including all of those listed below. l Read All Instructions – All safety and operating instructions must be read before operating the product. l Retain All Instructions – All safety and operating instructions must be retained for future reference. l Heed All Warnings – All warnings on the scanner and those listed in the operating instructions must be adhered to. l Follow All Instructions – All operating and scanner usage instructions must be followed. 1 Heat – The scanner must be situated away from any heat sources such as radiators, heat registers, stoves, or other products that produce heat. 2 Ventilation– Slots and openings in the scanner are provided for ventilation. They ensure reliable operation of the product, keeping it from overheating. These openings must not be blocked nor covered during operation. Keep air openings free of lint, hair, fluff, etc. This product should not be placed into a rack unless proper ventilation is provided through following the manufacturer’s recommended installation procedures. 3 Biosafety Procedures – Please refer to your institution’s biosafety policies and procedures regarding the proper handling of tissue and other potentially hazardous materials in connection with the use of this device. 4 Water and Moisture – Do not use the scanner near water—for example, near a water bath, wash bowl, kitchen sink, or laundry tub; in a wet basement; or near an open pool of water or the like. If the scanner becomes wet, unplug it before touching it. 5 Environment – This device is intended for indoor use only. 6 Attachments – Do not use any attachments not recommended by the product manufacturer as they may cause hazards. Aperio GT 450 User's Guide, MAN-0391 Revision G 19 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction 7 Power Sources – The scanner must be operated from the type of power source indicated on the marking label and in the installation instructions. If you are not sure of the type of power supplied to your facility, consult your local power company. Check that the voltage setting matches the electrical supply voltage. 8 Grounding and Polarization – The scanner AC/DC adapter is equipped with a polarized AC plug with integral safety ground pin. Do not defeat the safety ground in any manner. 9 Cable Protection – Secure all external cables to avoid operator injury. 10 Power Cord Protection – Power supply cords must be routed so that they are not likely to be walked on nor pinched by items placed upon or against them. Pay particular attention to the cords at AC wall plugs and convenience receptacles and at the point where the cord enters the AC/DC adapter. The appliance coupler on the external AC/DC power supply is considered the mains disconnect device. 11 Lightning – For added protection for this scanner during a lightning storm, or when it is left unattended and unused for long periods of time, unplug it from the AC wall outlet. This will prevent damage to the product due to lightning and power line surges. 12 Power Overloading – Do not overload AC wall outlets, extension cords, or integral convenience outlets as this can result in a fire or electric shock hazard. 13 Operating Environment – Observe these basic safety requirements: l Do not operate the scanner out of doors. l Do not use where aerosol sprays are being used or where oxygen is being administered. l Do not let the scanner or its cables or accessories come into contact with surfaces which are too hot to touch. l Do not place anything on top of the scanner. 14 Object and Liquid Entry – Never push objects of any kind into the scanner through openings as they may touch dangerous voltage points or short-out parts that could result in a fire or electric shock. Never spill liquid of any kind on the scanner. 15 Accessories – Do not place the scanner on an unstable cart, stand, tripod, bracket, or table or the scanner may fall, causing serious injury and damage to the product. Any mounting of the scanner needs to follow manufacturer’s installation instructions. Do not place anything on top of the scanner. WARNING: Use of this equipment adjacent to or stacked with other equipment should be avoided because it could result in improper operation. 16 Moving – Use care if you must slide the scanner around the workbench. If you need to pick up the scanner to move it off the workbench, contact Leica Biosystems Technical Services. Moving the scanner may void the applicable product warranty—contact Leica Biosystems for advice. 17 Servicing – Refer all servicing to qualified service personnel. Aperio GT 450 User's Guide, MAN-0391 Revision G 20 Copyright © 2024 Leica Biosystems Imaging, Inc.  1 Introduction 18 Damage Requiring Service – Unplug the scanner from the wall AC outlet and refer servicing to qualified service personnel under the following conditions: l When the AC cord or AC/DC adapter is damaged. l If liquid has been spilled or objects have fallen into the scanner. l If the scanner has been exposed to rain or water. l If the scanner does not operate normally (when you are following operating instructions). l If the scanner has been dropped or damaged in any way. l When the scanner exhibits a distinct change in performance. This indicates a need for service. 19 Replacement Parts – When replacement parts are required, be sure the service technician has used replacement parts specified by the manufacturer. Unauthorized substitutions may result in fire, electric shock, or other hazards and may affect product compliance. The objective in the Aperio GT 450 scanner has been specifically designed for this device. Do not replace it with another type of objective. WARNING: Use of accessories, transducers and cables other than those specified or provided by the manufacturer of this equipment could result in increased electromagnetic emissions or decreased electromagnetic immunity of this equipment and result in improper operation. Unauthorized substitutions may result in fire, electric shock, or other hazards and may affect product compliance. 20 Operational Check – Upon completion of any repairs to this scanner, ask the service technician to perform operation checks to determine that the product is in proper operating condition. 21 Cleaning – Apply the recommended cleaning solution to a clean cloth to clean the equipment. Do not apply cleaning solutions directly to the equipment. Aperio GT 450 User's Guide, MAN-0391 Revision G 21 Copyright © 2024 Leica Biosystems Imaging, Inc. 2 Aperio GT 450 overview In this section: Theory of operation 23 Scanner overview 23 Touchscreen interface overview 25 Help videos and guides 27 Key features 28 Aperio GT 450 User's Guide, MAN-0391 Revision G 22 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview This chapter provides an overview of the Aperio GT 450 Scanner. Theory of operation The Aperio GT 450 scanner contains an automated slide loader subsystem which is designed to process standard 1" × 3" (2.54 cm × 7.62 cm) microscope slides. The scanner automatically loads the slides from the slide racks that the technician loaded into the scanner carousel. The slides are moved onto the scanning stage based on the automated processing and queueing of the racks. Once the slide is on the stage, the imaging process begins with capturing the macro image, automatically detecting tissue, automatic placement of initial focus points (and reference stripe location), capturing the label image (including any barcode), and the image acquisition is initiated. The tissue finding and scanning processes are automated without any need for the user to manipulate the slides being processed. If there is a need based on the quality review of the scanned image or tissue finder results, the device has the capability to reinitiate the scan with a different user selected mode (entire slide scan). The device images the slide using transmitted light from the Koehler Light Source through the specimen to the optical path (including the objective, tube lens, apertures, and mirrors). At this point the light travels to the line scan camera where the imaging data is captured and processed. The Aperio GT 450 scanner acquires images in stripes, as the motion subsystem moves the slide across the Koehler and optical path. These stripes of data are processed in the control VPU and sent to a database for use in later pathology workflow steps. Once the slide has finished scanning, it is automatically removed from the stage, returned to the slide rack, and the next slide is loaded. Scanner overview This section describes the parts of your scanner used in daily operation. Front Back Aperio GT 450 User's Guide, MAN-0391 Revision G 23 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview The following section describes the main scanner elements: Element Use More details Touchscreen View rack statuses, set rack options, and view other information. See Touchscreen interface Interface overview (on page 25). Carousel The carousel contains 15 rack slots. After you load racks in the See Carousel rotation (on carousel, the carousel rotates the racks to the scanning area and page 42). the rack loading area. Rack loading Provides access to the carousel to load and unload slide racks. This See Load racks in the area area is also referred to as the “front-six,” because the rack loading carousel (on page 44). area gives you access to six rack slots at a time. Safety light The infrared safety light curtain detects objects in the rack loading See Carousel rotation (on curtain area. The carousel only rotates when the safety light curtain area is page 42). clear. Status lights The status lights are located below the rack slots in the rack loading See Check scan status (on area. They indicate the status of the front-six racks and the page 57). scanner. The colors of the status lights match the colors of the rack See Red blinking lights on status Legend on the touchscreen interface. carousel (on page 95). Fan filter The fan filter is located on the back of the scanner. Clean the fan See Clean the fan filter (on filter at least every six months. You can insert the fan filter from the page 88). left side, the right side, or the top (requires a different fan filter holder). Turn the scanner on and off The On/Off switch is located on the right side of the scanner near the back. l On position = l Off position = Aperio GT 450 User's Guide, MAN-0391 Revision G 24 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview When you are turning the scanner off and on, follow these procedures according to the situation: l Shut down the scanner (on page 75). l Restart the scanner after interior maintenance (on page 91). l Perform a safe restart after an error (on page 96). Touchscreen interface overview The touchscreen interface is where you view the scan status and interact with the scanner. Tap an area on the touchscreen interface to view information or perform an action. Home screen The Home screen (Carousel view) is where you view rack and slide status. The buttons along the top enable you to access other areas, such as Help and Maintenance. Aperio GT 450 User's Guide, MAN-0391 Revision G 25 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview The following section describes the Home screen elements. Element Icon Use More details Rack status not The Legend defines colors and icons that Check scan status (on page 57) legend applicable indicate rack status. Status colors appear on the rack slot positions on the touchscreen interface, and on the status lights in front of the carousel. Note: the Legend is for information only and is not interactive. Scanner Tap Stats to view scan statistics, such as the Scan statistics (on page 66) statistics number of slides or racks scanned within a certain time period. Maintenance Tap Maintenance if you need to restart the Serial number and firmware version scanner, or view the serial number and the (on page 74) hardware and firmware versions of your Shut down the scanner (on page scanner. 75) Restart the scanner (on page 77) Help Tap Help to view training and maintenance Help videos and guides (on page videos, or access an online version of the quick 27) reference guide. Carousel and not Use the carousel and rack position on the Carousel rotation (on page 42) rack status applicable touchscreen interface to: Check scan status (on page 57) l View the rack status, indicated by the color of the rack position. l Tap a rack position to select the rack. l Apply an action to the selected rack, such as Priority or Rotate (described next). Rack actions Select a rack and tap Priority to move the rack Priority scanning (on page 46) Note: Rack actions to the top of the scan queue. appear when you select a rack. Select a rack and tap Rotate to rotate the rack Rotate a rack to the rack loading to the rack loading area. area (on page 55) Rack settings Select a rack and tap Settings to select and Scan entire slide for whole rack (on Note: Rack settings apply a rack setting. page 63) appear when you Change the scan magnification for select a rack. a rack of slides (on page 47) Aperio GT 450 User's Guide, MAN-0391 Revision G 26 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview Element Icon Use More details Scan a rack of slides using z-stack scanning (on page 49) Scan a rack of slides using Extended Focus (on page 52) Login, Logout Tap Login to open a keypad and enter your Log in and log out of the scanner pass code to access the scanner. (on page 42) Tap Logout to lock access to the scanner touchscreen interface. Rack View Select a rack and tap Rack View to view the View slide status for a rack (on scan status for each slide in the rack. page 59) Slide View Select a rack and tap Slide View to view macro View macro images of scanned images for each slide. slides (on page 60) Rack Order Tap Rack Order to view scan order and status View rack scan order (on page 60) of the racks. Help videos and guides You can access training videos and the Aperio GT 450 Quick Reference Guide directly from the touchscreen interface. We recommend that you watch the training videos before operating the scanner for the first time. 1 From the Home screen, press Help, and then tap a video or guide to view. Aperio GT 450 User's Guide, MAN-0391 Revision G 27 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview Key features This section describes some key features of your Aperio GT 450. Continuous load workflow You can continuously load new racks and unload completed racks without interruption. Scan magnification Custom optics for native 40x scanning magnification, with the option to scan slides at 20x or 40x magnification, depending on the needs of your organization or specific project. Automatic image quality check The Auto-Image QC automatically checks scan quality. If the scan status is green for “complete,” the scan and Image QC were successful. If there is a problem with the scan quality on a slide, the system alerts you. Manual Scanning The optional Manual Scan feature enables you to manually adjust the scan settings using the macro image from an automatic scan and re-scan a single slide without removing the slide from the scanner. Z-Stack scanning The optional Z-Stack scanning feature enables you to scan a glass slide at different focal planes along the vertical z- axis, and stack the images on top of each other to produce a 3D composite multiplane image. Extended Focus The optional Extended Focus feature enables you to scan a single composite image file with optimized focus. The Extended Focus scanning process uses z-stack layers to achieve optimal focus on the entire depth of tissue. The optimally-focused areas across the z-stack layers are identified and then fused into a single image. Image types supported The Aperio GT 450 creates SVS files or DICOM images. The .svs image format is the default. For information on enabling the optional DICOM feature, see the Aperio GT 450 DICOM Upgrade Guide. Slide types supported The scanner supports 1-inch x 3-inch (2.54-cm x 7.62-cm) glass slides (per ISO 8037/1). Coverslips Fully-cured coverslips are required. The Aperio GT 450 supports common industry coverslips used for 1 x 3 inch slides. Aperio GT 450 User's Guide, MAN-0391 Revision G 28 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview Slide racks supported Optimized and recommended for use with Leica HistoCore Spectra workstation racks (stainer and coverslipper), which include the Leica Universal Rack 30-slide capacity (part number 23RACKGT450). Sakura Prisma Stainer and Coverslipper Rack 20-slide capacity racks are also accepted. Slide capacity The maximum slide capacity depends on the racks used: l 20-slide racks – load up to 300 glass slides. l 30-slide racks – load up to 450 glass slides. Barcodes supported The Aperio GT 450 supports the following barcodes: l NW7 l QR Code l Data Matrix l Interleaved 2 of 5 l Code 39 l Code 128 l PDF417 l MicroPDF41 l Aztec For more information about barcodes, see the Aperio GT 450 Specifications. Optional Aperio Viewing Station We recommend using the Aperio Viewing Station for slide image viewing. The Aperio Viewing Station is optional and is not included in the Aperio GT 450 product. For more information, contact your Leica Biosystems representative. If you do not purchase the viewing station, we recommend using a calibrated monitor for optimal image viewing. For detailed viewing station and monitor specifications and requirements, see the Aperio GT 450 Specifications. Aperio GT 450 User's Guide, MAN-0391 Revision G 29 Copyright © 2024 Leica Biosystems Imaging, Inc.  2 Aperio GT 450 overview Aperio Digital Slide Viewers You can use the following viewers to view digital slides created on the Aperio GT 450 scanner: l Aperio ImageScope (version 12.4 or higher) – this product comes pre-installed on viewing workstations (if purchased). For the latest version of Aperio ImageScope, contact your Lab Manager or download the installation file from the Leica Biosystems website. You must have administrator privileges on your viewing station to install Aperio ImageScope. l Aperio WebViewer – this viewer is integrated with Aperio eSlide Manager. It is also available for LIS integration. Aperio GT 450 User's Guide, MAN-0391 Revision G 30 Copyright © 2024 Leica Biosystems Imaging, Inc. 3 Slide preparation In this section: Slide preparation overview 32 Slide preparation checklist 33 Tissue preparation 33 Staining 33 Slide preparation guidelines 34 Coverslips 35 Labels 35 Barcodes 36 Slide quality control 36 General guidelines for slide preparation 37 Aperio GT 450 User's Guide, MAN-0391 Revision G 31 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation Slide preparation overview This section contains tips on best practices for slide preparation for your Aperio GT 450 scanner. To ensure the best possible quality and continued peak operation, it is crucial to ensure slides and scanner components are clean and free of obstructions. The scanner is designed to safeguard the handling of slides throughout the entire scanning process. The product features numerous sensors and mechanisms to detect the proper handling of slides. Should any of these safeguards detect an unexpected outcome, the device will safely halt ensuring the slide is protected from damage throughout the process. When preparing slides for scanning, you must use the procedures required by the vendors of the stains and reagents you are using. Well prepared slides are crucial to a successful scan. After reading this section, if you have questions about whether your slide preparation techniques will provide good scan quality, contact Leica Biosystems Technical Services for advice. Always consult the material safety data sheets for the stains and re-agents you are using, as well as your institution’s biosafety policies and procedures regarding safely handling biological materials. It is the responsibility of the lab to verify the quality of the tissue preparation, the physical characteristics of the slide, and the staining quality. CAUTION: Ensure that the slides and racks you use meet the Aperio GT 450 specifications. For details, see the Aperio GT 450 Specifications. Do not modify racks or attach labels to them. Aperio GT 450 User's Guide, MAN-0391 Revision G 32 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation Slide preparation checklist Slides Slide labels l Slides stained according to manufacturer’s instructions. l Only one label per slide l Slides are clean. Wipe with clean lint-free cloth (no chemical l Labels do not extend past edges of the cleaners). slide. l No dirt, fingerprints, markings, writing, extra mounting media, l Labels do not cover any tissue. broken slides, chips, or scratches. l Labels are not under the coverslip. l Slides are fully cured (not “wet”). l Labels are firmly attached. l No mounting media around slide edges. l Labels are not applied to the bottom l No tissue on coverslip edges or overlapping the label. surface of the slide. l No stain residue around slide edge. Coverslips Barcodes l Fully cured coverslip in place. Must use coverslip. l Barcode labels meet same application l Minimal mounting media used. requirements as slide labels l No air pockets under coverslip. l Barcode labels applied to the slide label region. l Coverslip is not hanging over the side of the slide. l Only one barcode label per slide. l Only one coverslip per slide. l Use only supported barcode labels. l Use only high-quality printed barcode labels. Tissue preparation For optimal scanner performance, slides must be well-prepared. Staining Reproducibility of the slide staining is important for consistent and accurate diagnosis. l Make sure that the variations of the staining process are controlled and eliminated to the greatest extent possible. l Use appropriate morphological studies and controls as specified in the reagent manufacturer’s instructions. l Avoid excessive stain residue, as this may affect scanner performance. Aperio GT 450 User's Guide, MAN-0391 Revision G 33 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation Slide preparation guidelines Many scanning issues are avoided by checking slide quality. Ensure the following: l Slides are very clean and in good condition. Wipe them with a clean cotton cloth (don’t use chemical cleaners). Make sure the slides have no dirt, no fingerprints, no markings, no writing, no extra mounting media, no broken slides, no chips, no scratches, etc. l Slides are fully cured (not “wet”). l All slides have coverslips. Coverslips are required when using the Aperio GT 450 scanner. l There is no mounting adhesive around the edges of a slide. This can cause it to stick or catch in the scanner stage area. CAUTION: Do not attempt to scan damaged or broken slides, as doing so may damage the scanner. Tissue placement Place the tissue in the middle of the slide, a distance from the edges of the slide, the label, and any other markings. Ensure the following: l The coverslip covers all the tissue. l The label does not cover any tissue. The example below shows minimum distances from the side and bottom edges of a 26mm x 76mm (1" x 3") slide. For more details on slide specifications, see Slide types supported (on page 28). Fixing slide preparation errors Some physical problems of a slide can be resolved by cleaning the slide with a lint-free cloth or trimming the sides with a razor blade. Permanent problems with a slide may require the preparation of a new slide. Aperio GT 450 User's Guide, MAN-0391 Revision G 34 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation Coverslips Coverslips are required when using the Aperio GT 450. They must be fully-cured or have completed the on-board drying process for an automated coverslipper, such as the Leica Biosystems HistoCore SPECTRA Coverslipper. The Aperio GT 450 supports common industry glass or plastic coverslips used for 1-inch x 3-inch (2.54 cm x 7.62 cm) slides. See the Aperio GT 450 Specifications for coverslip specifications. (Glass coverslips are recommended). The quality of the scan is affected by the condition of the coverslip. l Keep the mounting media that attaches the coverslip to the slide to a minimum. Excess mounting media makes it hard for the tissue finder to distinguish between actual tissue and the mounting media. l Make sure there are no air pockets under the coverslip. l For best results, do not mark or write on the coverslip. l The coverslip must not hang over the side of the slide. l Make sure there is only one coverslip attached to the slide. l Wipe the coverslip clean of dust and fingerprints before loading the slide in the scanner. Labels The Aperio GT 450 supports common industry labels used for 1 x 3 inch (2.54 cm x 7.62 cm) slides. Improperly applied slide labels can cause slides to jam. Ensure the following: l Do not apply multiple labels to the same slide—this can cause the slide to exceed the specification for slide thickness. See the Aperio GT 450 Specifications for slide specifications. l Labels do not extend past the edges of the slide and do not cover any tissue. l Labels are not underneath coverslips. l Labels are firmly attached. l Do not apply labels to the bottom surface of the slide. l Always load slides into the rack with the label facing out and up, as shown in Load slides in the rack (on page 43). Aperio GT 450 User's Guide, MAN-0391 Revision G 35 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation The examples below show a slide with multiple labels that cause the slide to exceed the allowable maximum thickness, and a slide with the label peeling away: Barcodes For a list of supported barcodes, see Barcodes supported (on page 29). For best performance, we recommend using barcode labels that have a white background and black ink. Improperly applied slide barcodes can cause slides to jam. Ensure the following: l Barcode labels meet the same application requirements as slide labels. See Labels (on page 35). l Apply barcode labels to the slide label region. l Ensure a minimum of 0.5 mm between each side of the barcode and the edge of the label. l Apply only one barcode label per slide. l Use only supported barcode labels. See Barcodes supported (on page 29). l Use only high-quality printed barcode labels. Slide quality control Look for these issues in prepared slides. Issue What to look for Sample Issues Labels Overhanging (size of label), peeling, multiple (covering tissue, barcodes), placement (label region), print quality and supported barcode type Coverslips Overhanging (skewed), lifting, multiple, bubbles, excessive mounting media (edges of slide, bottom), wet Aperio GT 450 User's Guide, MAN-0391 Revision G 36 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation Issue What to look for Sample Issues Cleanliness Paraffin (slide transportation, sorting, handling), pen marks, fingerprints, dust/debris Tissue Thickness, size and proximity to other tissue, placement (away from edges), folds, chatter Staining Intensity (too dark or too light), overstaining, contamination General guidelines for slide preparation To ensure the best possible quality and continued peak operation, it is crucial to ensure slides and scanner components are clean and free of obstructions. Examples of such obstructions are a) Debris on slides (on top and under slide), b) Overhanging labels and coverslip, c) Excessive mounting media. The scanner generates high magnification images with large field of view (FOV) at a high speed. To ensure good Image Quality, the slide needs to maintain its height on the stage while scanning and tissue/slide is flat within the FOV. If either of these fail, the sample will be out of focus or have uneven focus. Here are a few examples of poor sample preparation that may cause slide handling errors in a scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G 37 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation 1 An overhanging or skewed coverslip may lead to slide loading and unloading errors as the slide is loaded to or from the scanning stage. It may also cause the slide to not sit firmly or flat in the slide tray and could lead to a physical shift during the scan process. 2 An overhanging label may lead to slide loading or unloading errors. Residue from the overhanging label may also transfer to the pusher assembly leading to slide loading/unloading errors for subsequent slides until the residue is cleaned. 3 Excessive mounting media around slide edges may lead to debris build up within the slide racks slots. This may cause slides to become affixed within the slide rack. Mounting media around the slide edges may also lead to slide load or unload errors. Aperio GT 450 User's Guide, MAN-0391 Revision G 38 Copyright © 2024 Leica Biosystems Imaging, Inc.  3 Slide preparation 4 When numerous slides with excessive mounting media are scanned over time, there may be mounting media buildups on the tray opening leading to a higher chance of slide loading or unloading errors. Debris on the bottom of the slide may create a catch point during the slide load or unload process leading to slide handling errors. Label on the backside of a slide may also create a catch point during the slide load or unload process leading to slide handling errors. Place the tissue in the middle of the slide, a distance from the edges of the slide, the label, and any other markings. Ensure the following: The coverslip covers all the tissue and the label does not cover any tissue. The example below shows minimum distances from the side and bottom edges of a 26 mm x 76 mm slide. Aperio GT 450 User's Guide, MAN-0391 Revision G 39 Copyright © 2024 Leica Biosystems Imaging, Inc. 4 Scan slides In this section: Scanning concepts 41 Log in and log out of the scanner 42 Load slides in the rack 43 Load racks in the carousel 44 Priority scanning 46 20x magnification scanning 47 Z-Stack scanning 49 Extended Focus scanning 52 Rotate a rack to the rack loading area 55 Unload racks from the carousel 56 Check scan status 57 Scan entire slide for whole rack 63 Image quality control for histotechnicians and pathologists 64 Scan statistics 66 Manual Scanning 67 Aperio iQC Software Module Notifications 72 Aperio GT 450 User's Guide, MAN-0391 Revision G 40 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides This chapter shows you how to scan slides. Scanning concepts This section provides basic concepts on the scanning workflow and the carousel rotation features. We recommend reviewing this section before using the scanner. Scan workflow The Aperio GT 450 is a continuous load scanner. You can continually load new racks and unload completed racks, as shown below: Aperio GT 450 User's Guide, MAN-0391 Revision G 41 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Carousel rotation Review the following information to understand the carousel rotation and safety features. WARNING: Keep hands away from the carousel and pinch point areas when the carousel is rotating. Log in and log out of the scanner Your Lab Administrator sets up the scanner pass codes and timeout period for your lab using the Aperio GT 450 Aperio GT 450 SAM client application software. You need to log into the scanner to interact with the touchscreen interface. You do not need to log in to load and unload racks for scanning. As a security measure, the scanner logs you out after a period of time and requires you to enter the passcode. 1 From the touchscreen interface, tap Login. Aperio GT 450 User's Guide, MAN-0391 Revision G 42 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 2 When the keypad screen appears, enter your 5-digit passcode. To log out: Logging out locks the touchscreen interface until a valid passcode is entered. 1 From the touchscreen interface, tap Logout. Load slides in the rack For a successful scan, ensure you use the correct orientation of the slides and the rack. To load slides in the rack: 1 Position the rack so the Leica logo and “SIDE UP” face up. 2 Insert each slide as shown with the label facing out and up. 3 Push each slide into the rack until the slide touches the back of the rack, ensuring each slide is fully inserted into the rack. CAUTION: Use caution when loading slides into a rack to prevent improper slide orientation or damaged slides. Never use damaged slides in the scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G 43 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Load racks in the carousel Slides are scanned in the order you load them. 1 Place the loaded rack into an open slot in the carousel. 2 Push the rack forward until it stops and you hear a click. When the rack is fully inserted, there is a “click” sound, and the rack position on the Home screen turns light blue. 3 Continue loading racks as needed or until you fill the front six rack slots. Aperio GT 450 User's Guide, MAN-0391 Revision G 44 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 4 When you’ve finished loading the first set of racks: l The first rack is rotated to the scan area. l Any empty rack slots are rotated to the loading area. WARNING: To prevent injury, keep hands away from the carousel and pinch point areas when the carousel is rotating. Never manually rotate the carousel unless you are performing maintenance and power is off to the scanner as discussed in Chapter 5: Maintenance. 5 When a rack is scanned successfully, the rack status turns green. 6 You can unload any completed racks and continue loading new racks. If the completed rack is in the back of the carousel, you can rotate it to the rack loading area. See Rotate a rack to the rack loading area (on page 55). Aperio GT 450 User's Guide, MAN-0391 Revision G 45 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Priority scanning Use Priority to move a rack to the front of the scan queue. You can apply the Priority option on up to three racks at a time. 1. Tap the rack position. The Priority option appears when you select the rack position. 2. Tap Priority. The priority icon and scan queue number appear on the rack position. Aperio GT 450 User's Guide, MAN-0391 Revision G 46 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 20x magnification scanning You have the option to scan slides at 20x or 40x magnification, depending on the needs of your organization or specific project. This section contains instructions for performing rack-level scans at 20x or 40x magnification. Your Aperio GT 450 scanner is initially set to scan all racks using 40x magnification. If you want this scanner to scan using 20x magnification by default, your administrator must turn on the DEFAULT TO 20X SCANNING option in SAM. For more information, see the Aperio GT 450 IT Manager and Lab Administrator Guide. Change the scan magnification for a rack of slides For each Aperio GT 450 scanner, your Lab Administrator has the option of changing the default scan magnification from 40x to 20x within the SAM software. This section shows how to override the default during scanning, and manually change the scan magnification for a rack of slides. This procedure describes how to scan a rack of slides at 40x magnification, when the default scan magnification is set to 20x. (If the default scan magnification is set to 40x, use the same process to scan a rack of slides at 20x magnification.) To scan a rack of slides at 40x when the scan magnification is set to 20x, follow these steps: 1 Load the rack with only the slides that you want to scan at 40x. 2 Tap the rack position that contains the slides you just loaded. 3 Tap Settings , and then tap Scan at 40X. Aperio GT 450 User's Guide, MAN-0391 Revision G 47 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides The Scan at 40x option is selected, and 40x appears on the rack position. During scanning, the Rack View indicates the scanner is scanning this rack at 40x magnification. Aperio GT 450 User's Guide, MAN-0391 Revision G 48 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Z-Stack scanning With the optional Z-Stack scanning feature, the scanner can create an image of multiple layers of the slide tissue scanned at different focal depths, creating a 3D composite multiplane image that you can visually navigate through much as a microscope user can navigate through different tissue focal depths by using the microscope objective fine and coarse adjustments. This ability to create a 3D image is called “z-stack" scanning. Pathologists can review slide samples at varied “heights” using a software focus adjustment, comparable to the fine- focus knob of a conventional light microscope. The Z-Stack scanning feature is specifically useful in cytology and dermatopathology. For all types of scanning, the scanner determines the layer within the tissue that provides the optimal focus—this is called the best focus layer. For z-stack scanning, by default the best focus layer is placed in the middle of the z-stack, with an equal number of layers above and below it. Scan a rack of slides using z-stack scanning For each scanner in Aperio GT 450 SAM, your Lab Administrator sets the default number of layers and the distance between layers (in microns) that are used for z-stack and extended focus scanning.​ You can adjust these settings on the scanner console for each rack of slides you scan. Note the following when using Z-Stack scanning: l The Auto Narrow Stripe feature is not used to scan z-stack images. If the Auto Narrow Stripe scanning feature is turned on in SAM, the system disables this feature during z-stack scanning. l When using Z-stack scanning, the scan time and file size can increase, based on specified number of layers and the distance between layers. To scan a rack of slides using z-stack scanning, follow these steps: 1 Load the rack with only slides that you want to scan as z-stack images. You should load slides of similar tissue types in the same rack. This is because you choose the number of layers to use depending on the type of tissue you are scanning. Perform steps 2 and 3 below immediately after loading the rack in step 1 to ensure the scanner does not start scanning using the standard (non-z-stack) scanning process. 2 Tap the rack position that contains the slides you just loaded. 3 Tap Settings , and then tap Z-Stack. (If your scanner has the optional Extended Focus feature installed, the setting will include "Z-Stack and Extended Focus." Aperio GT 450 User's Guide, MAN-0391 Revision G 49 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides The Z-STACK OPTIONS screen appears with the default Number of layers and Layer separation (microns) that your Lab Administrator previously set in SAM. (If your scanner has the optional Extended Focus feature installed, then the Extended Focus options also appear on this screen.) Aperio GT 450 User's Guide, MAN-0391 Revision G 50 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 4 Follow these steps to adjust the z-stack scan settings for the rack you are scanning: a To adjust the Number of layers, tap the Minus and Plus buttons until you reach the desired number of layers. You can also use the number pad to enter the desired values directly. Tap inside the Number of layers field to activate the keypad. (Note that the number of layers must be an odd number.) b To adjust the Layer separation, tap the Minus and Plus buttons until you reach the desired number (in microns). As described in the step above, you can also use the number pad to enter the desired values directly. 5 Tap Apply Z-Stack Settings to start scanning the specified rack of slides. After you tap Apply Z-Stack Settings, the Z-Stack icon appears on the rack position. During scanning, the Z-Stack icon appears in Rack View to indicate the scanner is scanning this rack using the z-stack process. View z-stack images To view the 3-dimensional z-stack images, you must use a digital slide viewer that supports z-stack viewing, such as Aperio WebViewer (versions 1.2 and later), Aperio ImageScope, or a viewer of your choice that supports z-stack viewing. Aperio GT 450 User's Guide, MAN-0391 Revision G 51 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Extended Focus scanning The optional Extended Focus feature enables you to scan a slide to generate a single composite image with optimized focus and a greater depth of field than an image of a scanned single layer or any single layer in a set of z-stack images. The Extended Focus process uses scanned z-stack layers to achieve optimal focus on the entire depth of the tissue. The Extended Focus process identifies the optimally focused areas across the z-stack layers and fuses those layers into a single, composite image. This feature is particularly useful in applications where it is preferable to view all cells in focus in a single-layer image. An extended focus image has a smaller file size than the set of z-stack images from which it is generated. This smaller file size helps increase file transfer speed during collaborative activities, and also requires less storage space. The single composite image file can be compatible in external image analysis software algorithms when a set of z-stack layers might not be. Similar to the z-stacking feature, you set Extended Focus for an entire rack of slides. To use the Extended Focus feature, you must have the Extended Focus option installed on your scanner. Scan a rack of slides using Extended Focus For each scanner in Aperio GT 450 SAM, your Lab Administrator sets the default number of layers and the distance between layers (in microns) that are used for z-stack and extended focus scanning.​ You can adjust these settings on the scanner console for each rack of slides you scan using the Extended Focus feature. To scan a rack of slides using the Extended Focus feature, follow these steps: 1 Load the rack with only slides that you want to scan as extended focus (EF) images. You should load slides of similar tissue types in the same rack. This is because the number of layers and the distance between the layers you choose for Extended Focus are applied to all the slides in the rack. The settings you use depend on the type of tissue you are scanning. Perform steps 2 and 3 below immediately after completing loading the rack in step 1 to ensure the scanner does not start scanning using the standard (non-z-stack) scanning process. 2 Tap the rack position that contains the rack of slides you just loaded. 3 Tap Settings , and then tap Z-Stack and Extended Focus. Extended Focus appears as an option only if the Extended Focus feature is installed on your scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G 52 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 4 The Z-STACK AND EXTENDED FOCUS SETTINGS screen appears with the default Number of layers and Layer separation (in microns) that your Lab Administrator previously set in Aperio GT 450 SAM. 5 Follow these steps to adjust the Z-STACK AND EXTENDED FOCUS SETTINGS for the rack of slides you are scanning: a To adjust the Number of layers, tap the Minus and Plus buttons until you reach the desired number of layers. You can also use the number pad to enter the desired values directly. Tap inside the Number of layers field to activate the keypad. (Note that the number of layers must be an odd number.) b To adjust the Layer separation, tap the Minus and Plus buttons until you reach the desired number (in microns). As described in the step above, you can also use the number pad to enter the desired values directly. Aperio GT 450 User's Guide, MAN-0391 Revision G 53 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 6 Under Output Type, select Extended Focus if it is not already selected. (Your Lab Administrator may have already set this option as a default in Aperio GT 450 SAM.) Note that Extended Focus image file names are appended with "_EF." To generate both a z-stack image and an extended focus image, you can also select Z-Stack. 7 Tap Apply Settings to start scanning the specified rack of slides. 8 After you tap Apply Settings, the Z-stack icon appears on the rack position. . During scanning, the Z-stack icon appears in Rack View to indicate the scanner is scanning this rack using the z-stack process. Aperio GT 450 User's Guide, MAN-0391 Revision G 54 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Rotate a rack to the rack loading area The main reason for using the rotate feature is to rotate a completed rack to the loading area. If you try to rotate the rack that is currently being scanned, the system asks you for confirmation before continuing. To rotate a rack to the rack loading area: 1 Tap the rack position. The Rotate option appears when you select the rack position. 2 Tap Rotate to rotate the rack to the loading area. 3 After the rack is rotated to the front, you can remove the rack. (The status light in the loading area blinks to indicate the location of the rotated rack). Aperio GT 450 User's Guide, MAN-0391 Revision G 55 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Unload racks from the carousel CAUTION: Use caution when unloading racks from the carousel and when unloading slides from a rack to prevent damaged slides or operator injury. Never use damaged slides in the scanner. To unload racks from the carousel: 1 Make sure all the slides in the rack were successfully scanned (the rack status is green). If the rack status is orange, see Rack warnings (on page 61). 2 If the rack is in the loading area, carefully remove the rack from the rack slot. 3 If the rack is not in the loading area, you can rotate it to the front: a Tap the rack position. Aperio GT 450 User's Guide, MAN-0391 Revision G 56 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides b Tap Rotate. If you try to rotate the rack that is currently being scanned, the system asks you for confirmation. 4 After the rack is rotated to the loading area, you can remove the rack. Check scan status This section describes the different ways to check scan status. CAUTION: If you must remove a rack before all slides in the rack are scanned, note the rack and slide status. After removal, the rack’s scan status is no longer available. Aperio GT 450 User's Guide, MAN-0391 Revision G 57 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Check rack status To check the scan status of a rack: 1 Check the rack position status color against the Legend. The rack statuses are: Empty Rack is empty and available for use. Complete All slides in the rack scanned successfully and passed image QC. Scanned images are saved to the specific location. Scanning The rack is currently being scanned. Waiting to Scan The rack is loaded successfully and is waiting to scan. Warning There is an issue with the rack, or one or more slides in the rack. If you get a rack warning, see Rack warnings (on page 61). Priority The rack is set for priority scanning. See Priority scanning (on page 46). Rack error on current scan If there is an error on at least one slide in the rack that is currently scanning, the rack position pulses blue and orange. See Rack warnings (on page 61). Aperio GT 450 User's Guide, MAN-0391 Revision G 58 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides View slide status for a rack To view the status for slides in a rack: 1 Tap the rack position on the touchscreen interface. 2 Tap the Rack View icon. Slide status colors correspond to the Legend: The slide is scanning. The slide is waiting to scan. The slide slot is empty. The scanned image was successfully transferred to the specific image location. No scanned image created. See Rack warnings (on page 61). The slide scanned successfully, passed image QC, and was transferred to the specific image location. The slide has a scan warning. See Rack warnings (on page 61). Aperio GT 450 User's Guide, MAN-0391 Revision G 59 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides View macro images of scanned slides To view a macro image of a scanned slide: 1 Tap the rack position on the touchscreen interface. 2 Tap the Slide View icon to view individual images for the selected rack. l Tap and to view other slides in the rack. l If any tissue is outside the scan area, you can tap Scan Entire Slide to rescan the whole slide. l If there is an error scanning the slide, a message appears in the box. See Slide errors and solutions (on page 117). View rack scan order 1 Tap the Rack Order icon to show the scan order of the racks. l Racks in the list appear in the scan order. l The scan status is indicated for each rack. l In the example, rack 3 is set as priority. Aperio GT 450 User's Guide, MAN-0391 Revision G 60 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Rack warnings A rack has a warning if the status color is orange or if the rack position is pulsating blue and orange. To check the rack for errors: 1 Tap the rack position you want to check. 2 If there is a problem with the rack, a message appears similar to the example below. 3 Take note of the error code and message. For information and steps to resolve the rack error, go to Rack warnings and solutions (on page 115). Aperio GT 450 User's Guide, MAN-0391 Revision G 61 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 4 If there is a problem with one or more slides in the rack, a warning icon appears next to the slide in Rack View: The scanner created an image, but there is an error. An error prevented the scanner from creating an image. An error occurred when transferring an image to the designated storage location A barcode is missing or invalid. (This issue only occurs if the Aperio GT 450 SAM is configured to require barcodes.) In the example below, slide 7 in the selected rack has a warning. 5 Tap the Slide View icon to view the macro image for the slide that has a warning. 6 Take note of the error code and message. For information and steps to resolve the error, go to Slide errors and solutions (on page 117). Aperio GT 450 User's Guide, MAN-0391 Revision G 62 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Scan entire slide for whole rack This feature enables you to scan the full slide area for an entire rack of slides. 1 Load only the slides that require a full slide area scan in the rack. 2 Load the rack in the carousel. 3 Tap to select the rack position. 4 Tap Settings , and then tap Scan Entire Slide. The Scan Entire Slide option is selected, and the Settings icon appears on the rack position: Aperio GT 450 User's Guide, MAN-0391 Revision G 63 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Image quality control for histotechnicians and pathologists After scanning slides, it is important to review the digital slides to make sure the images are of good quality. Ultimately, it is up to the pathologist to look at digital slides to verify they are of sufficient quality to perform their task. In addition, scanner operators should verify digital slide quality after scanning. The quality criteria that should be verified by scanner operators are: l that the entire tissue sample has been scanned, and; l that the tissue is in focus. Aperio scanners provide a macro image, a low resolution image of the entire slide, that also provides a green outline of the scanned image. Check that image for the quality criteria mentioned above. (For more details, see the next section, Check that the scanned image covers all slide tissue.) Re-scan any slides that failed to scan or failed the image quality review. For additional image quality assessment instructions, refer to the user guide for your viewer. Check that the scanned image covers all slide tissue The steps below describe how to check that the scanned image covers all of the slide tissue. 1 Tap the rack position on the touchscreen interface that contains the slide images you want to review. 2 In the right pane of the touchscreen interface, tap the Slide View icon to view the individual scanned images for the selected rack. (Tap and to navigate through the slides in the rack. You can view slide images only after the scanner has completed scanning the slide.) Aperio GT 450 User's Guide, MAN-0391 Revision G 64 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 3 The green box on the macro image indicates the scan area, as shown below. 4 Visually check the macro image to ensure all of the tissue is inside the scan area. Tissue that extends outside of the scan area is not included in the scanned image. Tissue is within the scan area Tissue is outside the scan area In the example below, all of the tissue is In the example below, tissue extends outside inside the scan area. This means all of the the scan area. This means some tissue is not tissue is included in the scanned image. included in the scanned image. 5 If any tissue is outside the scan area, you can tap Scan Entire Slide to rescan the whole slide. If your scanner has the optional Manual Scan feature, tap Manual Scan to adjust the scan area. For more details, see Manual Scanning (on page 67) Aperio GT 450 User's Guide, MAN-0391 Revision G 65 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Scan statistics The Aperio GT 450 provides scan statistics, such as the number of slides or racks scanned per day, per week, etc. Tap Stats to view statistics for the Last 7 Days, Last 12 Months, or Lifetime by Year. Aperio GT 450 User's Guide, MAN-0391 Revision G 66 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Manual Scanning If a scanned image has certain image quality issues, sometimes the best resolution is to re-scan the slide using a manual process. The optional Manual Scan feature enables you to manually adjust the scan settings using the macro image from the automated scan, and then re-scan a single slide without removing the slide from the scanner. Manual Scan overview You access the Manual Scan settings from the Slide View, which shows the macro image from the automated scan. The Manual Scan feature enables you to: l Adjust the bounding box that defines the scan area. l Add or remove focus points. l Verify that the calibration point is positioned correctly, and re-position it if needed. l Zoom into the macro image of the slide to access potential problem areas. Manual Scan user interface The example below shows the features of the Manual Scan user interface. Use Manual Scan to resolve an image quality issue If the scanner indicates a slide image has a quality issue, follow these steps to manually re-scan the slide: Aperio GT 450 User's Guide, MAN-0391 Revision G 67 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 1 On the touchscreen, tap the rack position that has the error. 2 Tap the Rack View icon to view the list of slides in the rack. Any slides with scan issues are colored orange, as shown below. 3 Tap the Slide View icon. The macro image for the first slide in the rack appears. Aperio GT 450 User's Guide, MAN-0391 Revision G 68 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 4 Tap the left or right navigation arrow (shown above) to display the problem slide, and then tap Manual Scan. The Manual Scan settings for the image open in the scanner console. Aperio GT 450 User's Guide, MAN-0391 Revision G 69 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides 5 Adjust the Manual Scan settings as needed, using the instructions in the following table. Refer to Manual Scan user interface (on page 67) as needed. To do this: Follow these steps: Adjust the scan area Drag any corner of the green bounding box to move or re-size the box to the desired position. Only the tissue inside the scan area is included in the scanned image. When you adjust the scan area, the system recalculates the number of focus points. Add or remove focus points The yellow dots in the scan area are focus points. The scanner calculates the number of focus points to present for use depending on the specified scan area. Add or remove focus points in the following ways: l Tap to open the Focus Point Density slider. Drag the Focus Point Density slider left for fewer focus points, and right for more focus points. l Tap a focus point to delete it. (There must a minimum of three focus points for a slide.) l Double-tap an area to add a focus point. l Drag to move a focus point to a new position. Locate and verify the placement of the To verify the calibration point: calibration point 1 Click the Focus View on Calibration Point button to bring the calibration point (the blue diamond) into view. 2 Ensure the calibration point is on a clear area of the slide that does not contain tissue and that is under the coverslip. (Note that the calibration point can be located outside the bounding box of the scan area.) 3 If necessary, drag it to a new position. Zoom in or out for a different view of the Pinch-to-zoom or tap the Zoom In and Zoom Out buttons macro image to adjust the view of the image. Zoom to the scan area Tap the Focus View on Scan Area button to bring the scan area into view. Tap the button again to return to thefull view. Aperio GT 450 User's Guide, MAN-0391 Revision G 70 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides To do this: Follow these steps: Reset to the default scan settings Tap the Reset to Default Settings button to erase your changes and return to the default settings, as determined by the scanner's tissue finder. 6 When you are satisfied with your settings, tap Submit. The rack is added to the scan queue, with the manual scan settings applied to designated slide. You can prioritize the rack in the scan queue by following the instructions in Priority scanning (on page 46) Aperio GT 450 User's Guide, MAN-0391 Revision G 71 Copyright © 2024 Leica Biosystems Imaging, Inc.  4 Scan slides Aperio iQC Software Module Notifications The Aperio iQC Software Module is a standalone software application intended to assist in identifying artifacts in whole slide images (WSIs) produced by the Aperio iQC Software Module scanner. If the Aperio iQC Software Module software identifies any specified artifacts on the scanned image, the rack status indicates an error and a message appears within the Slide View on the scanner's console, as shown in the example below. Your IT Administrator sets up the communication between your Aperio GT 450 scanner and the Aperio iQC Software Module. For more details, see the Aperio GT 450 IT Manager and Lab Administrator Guide and your Aperio iQC Software Module IT Administrator's Guide. For instructions on using the Aperio iQC Software Module, see your Aperio iQC Software Module User's Guide. Aperio GT 450 User's Guide, MAN-0391 Revision G 72 Copyright © 2024 Leica Biosystems Imaging, Inc. 5 Maintenance This chapter contains a maintenance schedule and procedures for maintaining your Aperio GT 450 scanner. Call Leica Biosystems Technical Services if you cannot perform a maintenance routine or if you discover a problem with your scanner. Before performing maintenance, we recommend you watch the maintenance videos available on the touchscreen. See Help videos and guides (on page 27). CAUTION: It is important that you perform the maintenance procedures listed below on a regular schedule. Not doing so could result in scanner misalignment which could impair image quality. In this section: Maintenance schedule 74 Serial number and firmware version 74 Shut down the scanner 75 Open the scanner cover and access the interior components 75 Recommended daily maintenance 77 Six month maintenance 82 Restart the scanner after interior maintenance 91 Transporting or moving the Aperio GT 450 92 Long term storage 92 Scanner Maintenance Log 93 Aperio GT 450 User's Guide, MAN-0391 Revision G 73 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Maintenance schedule To keep your Aperio GT 450 scanner in optimal working condition, follow these maintenance routines. Frequency Maintenance Task Procedure Daily Restart the scanner. Restart the scanner (on page 77) (Recommended) Inspect the Stage Slide Tray Inspect the stage slide tray Inspect the Pusher/Puller Inspect the pusher/puller Every Six Months 1 Clean the objective and Koehler Clean the objective and Koehler (on page 82) 2 Clean the stage slide tray Clean the stage slide tray (on page 85) 3 Clean the carousel Clean the carousel (on page 87) 4 Clean the slide racks Clean the slide racks (on page 90) 5 Clean the fan filter Clean the fan filter (on page 88) 6 Clean the touchscreen Clean the touchscreen (on page 91) 7 Clean the scanner cover Clean the scanner cover (on page 90) Once a year Schedule annual maintenance visit by Leica Call Leica Biosystems Technical Services. Biosystems Technical Services. Serial number and firmware version You need the serial number and current software version if you call Leica Biosystems Technical Support. Tap Maintenance on the touchscreen interface to view the serial number, firmware version, and other system information. Aperio GT 450 User's Guide, MAN-0391 Revision G 74 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Shut down the scanner Use this procedure when you need to turn off the scanner. 1 From the touchscreen interface, tap Maintenance. 2 Tap Shut Down Scanner. 3 After the touchscreen goes dark, turn the scanner off using the On/Off switch. Open the scanner cover and access the interior components Complete these steps to access the inside of the scanner for maintenance or troubleshooting. If you are performing maintenance on the scanner, you should first remove any racks from the carousel. Aperio GT 450 User's Guide, MAN-0391 Revision G 75 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 1 Shut down the scanner using the steps in Shut down the scanner (on page 75). 2 Grasp the cover using the handhold indentations: 3 Slide the cover out until it reaches the point shown below. 4 Rotate the VPU latch 180 degrees, as shown below. Aperio GT 450 User's Guide, MAN-0391 Revision G 76 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 5 Carefully pivot the VPU outward as shown. 6 You can now access the interior components of the scanner for maintenance or troubleshooting purposes. 7 When you are ready to close the VPU and slide the cover back into place, following the instructions in Restart the scanner after interior maintenance (on page 91). Recommended daily maintenance This section provides the recommended daily maintenance procedures. Restart the scanner l Frequency: daily, and as needed to resolve a scanner issue. Restarting the scanner initializes the controller and puts the camera, stage, and autoloader in their home positions. CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 1 Before restarting the scanner, ensure the following: l All racks are unloaded from the carousel. l There are no slides scanning. l The system does not have any errors displayed. Aperio GT 450 User's Guide, MAN-0391 Revision G 77 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 2 From the touchscreen interface, tap Maintenance and tap Restart Scanner. You can use the scanner again after initialization is complete and the Home screen appears. Restart the scanner l Frequency: daily, and as needed to resolve a scanner issue. Restarting the scanner initializes the controller and puts the camera, stage, and autoloader in their home positions. CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 1 Before restarting the scanner, ensure the following: l All racks are unloaded from the carousel. l There are no slides scanning. l The system does not have any errors displayed. Aperio GT 450 User's Guide, MAN-0391 Revision G 78 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 2 From the touchscreen interface, tap Maintenance and tap Restart Scanner. You can use the scanner again after initialization is complete and the Home screen appears. Inspect the stage slide tray l Frequency: daily, and as needed to resolve a scanner issue. Perform a visual inspection daily. Clean the slide tray if you observe debris. 1 Ensure there are no slides scanning and the touchscreen interface does not display any errors. 2 Open the scanner interior. For instructions, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G 79 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 3 Visually inspect the stage slide tray (shown in blue). If you see debris on the slide tray, clean it using a plastic scraper or edge of a blank glass slide, removing any mounting, debris, or high points. The stage slide tray is not removable. When cleaning it in place, do not bump the LED or objective (highlighted in red): 4 Gently wipe the slide tray from back to front with a lint-free cloth. Do not use solvents such as xylene and toluene to clean the stage slide tray, as these solvents can damage the stage, lens coatings, wiring, and other internal components. Using such chemical solvents may also cause issues with the stage coating, resulting in loading or focusing errors during the scanning process. 5 Wipe the slide tray again from back to front using a microfiber cloth. If necessary, use a gentle solvent such as denatured alcohol to remove any remaining residue. (To order a cleaning kit for this purpose, contact your Leica Biosystems representative. Aperio GT 450 User's Guide, MAN-0391 Revision G 80 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Inspect the pusher/puller l Frequency: daily, and as needed to resolve a scanner issue. Visually inspect the pusher and puller assemblies. If you see debris on surfaces that contact the slide, clean the pusher and puller. 1 Ensure there are no slides scanning and the touchscreen interface does not display any errors. 2 Open the scanner interior. For instructions, see Open the scanner cover and access the interior components (on page 75). 3 Inspect the pusher contact surfaces (shown in blue): 4 If the pusher contact points contain debris, wipe them using a plastic scraper or edge of a blank glass slide, removing any mounting, debris, or high points. 5 Use a lint-free cloth or alcohol wipes to clean the surfaces. Aperio GT 450 User's Guide, MAN-0391 Revision G 81 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 6 Inspect the puller contact surfaces (shown in blue): 7 If the puller contact surfaces contain debris, wipe them using a plastic scraper or edge of a blank glass slide, removing any mounting, debris, or high points. 8 Use a lint-free cloth or alcohol wipes to clean surfaces. Six month maintenance This section provides the six-month maintenance procedures. The purpose of the six month maintenance is to clean the scanner components. Clean the objective and Koehler l Frequency: at least every six months. CAUTION: When cleaning the scanner’s objective: l Do not remove the objective. l Only use the recommended cleaning cloth and solution on the objective. Otherwise, this may damage the objective and impact image quality. l Do not drag anything (including lens paper) across the lens surface using a lot of pressure. This may damage the lens. Aperio GT 450 User's Guide, MAN-0391 Revision G 82 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Materials needed As stated in the previous "CAUTION," use only the cleaning product specified below to clean the objective and Koehler. Using other products, including fiber-reinforced paper towels or other paper products, may leave debris deposits that can affect image quality. l Texwipe TX404 Absorbond Synthetic Wipers (lens cleaning wipes made of lint-free microfiber) l Laboratory gloves To clean the objective and Koehler: 1 Ensure the scanner is turned off. (For instructions, see Turn the scanner on and off (on page 24)). 2 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G 83 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 3 The objective and Koehler are shown below in blue: 4 Manually position the slide stage so that you have clear access to the objective, as shown below. CAUTION: The objective may move when you're cleaning it. 5 Wear gloves when cleaning the objective. Aperio GT 450 User's Guide, MAN-0391 Revision G 84 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 6 Clean the objective using small circular motions with the recommended lint-free microfiber cloth. 7 With a clean lint-free microfiber cloth, use the same technique described in step 6 to clean the Koehler. The Koehler is located below the objective, as shown circled in orange below. 8 If you are performing the entire six-month maintenance routine, continue to the next procedure. 9 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Clean the stage slide tray l Frequency: at least every six months. To clean the stage slide tray: Aperio GT 450 User's Guide, MAN-0391 Revision G 85 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 1 If the scanner is on, shut down the scanner by following the steps in Shut down the scanner (on page 75). 2 Open the cover and access the interior. a Open the scanner cover. b Rotate the VPU latch. c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). CAUTION: The stage slide tray is not removable. Do not attempt to remove it. Aperio GT 450 User's Guide, MAN-0391 Revision G 86 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 3 Gently wipe the slide tray (shown in blue) from back to front with a lint-free cloth. CAUTION: Be careful not to bump the LED or the objective (highlighted in red). 4 Wipe the slide tray again from back to front using a microfiber cloth. 5 If necessary, use a gentle solvent such as denatured alcohol to remove any remaining residue. 6 If you are performing the entire six-month maintenance routine, continue to the next procedure. 7 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Clean the carousel l Frequency: at least every six months. To clean the carousel: 1 If the scanner is on, shut down the scanner by following the steps in Shut down the scanner (on page 75). 2 Remove all slide racks from the carousel. Aperio GT 450 User's Guide, MAN-0391 Revision G 87 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 3 Use a cotton swab soaked in a cleaning solvent, such as denatured alcohol, to thoroughly clean the carousel. Be sure to clean inside the slide rack slots. CAUTION: When manually rotating the carousel to access the rack slots, grasp the area circled in green. Do not grasp the area circled in red. 4 Manually rotate the carousel to access the rear slide rack slots. 5 Repeat the cleaning steps until you have cleaned every slide rack slot. 6 If you are performing the entire six-month maintenance routine, continue to the next procedure. 7 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Clean the fan filter The fan filter is located on the back of the scanner. You may need to pivot the scanner to access the fan filter. l Frequency: at least every six months. To clean the fan filter: Aperio GT 450 User's Guide, MAN-0391 Revision G 88 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 1 If the scanner is on, shut down the scanner by following the steps in Shut down the scanner (on page 75). 2 Remove the fan filter by gripping the handle (shown in blue), and sliding it left, right, or up depending on your scanner’s configuration. 3 With the front of the filter up toward the faucet (as shown below), rinse the filter under warm water. 4 Shake off any excess water. 5 Dry the filter thoroughly with a clean cloth or a dryer. CAUTION: Ensure the fan filter is completely dry before inserting it in the scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G 89 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 6 When the filter is completely dry, slide it back into position. 7 If you are performing the entire six-month maintenance routine, continue to the next procedure. 8 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Clean the slide racks l Frequency: at least every six months. To clean the slide racks: 1 Check the racks for any damage or accumulated glass dust and mounting media. 2 If a rack is damaged, replace it immediately. 3 Use compressed air or a cleaning solvent to clean all grooves in the rack, ensuring the racks are clean and smooth. (We recommend Xylene cleaning solvent). 4 If you are performing the entire six-month maintenance routine, continue to the next procedure. 5 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Clean the scanner cover l Frequency: at least every six months. To clean the scanner cover: 1 Wipe the outer cover of the scanner with a damp cloth. 2 Immediately dry the cover using a dry cloth. Aperio GT 450 User's Guide, MAN-0391 Revision G 90 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Clean the touchscreen l Frequency: at least every six months. To clean the touchscreen: 1 Spray standard monitor cleaner directly on a clean non-scratch cloth. (Avoid spraying the touchscreen directly to ensure that no liquid gets inside the scanner). 2 Thoroughly wipe down the touchscreen with the cloth. 3 If you are ready to close the scanner cover and restart the scanner, follow the steps in Restart the scanner after interior maintenance (on page 91). Restart the scanner after interior maintenance Follow these steps to restart the scanner after you have accessed the scanner interior. 1 Pivot the VPU into the closed position. 2 Secure the VPU in place by rotating the VPU latch 180 degrees. Aperio GT 450 User's Guide, MAN-0391 Revision G 91 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance 3 Slide the scanner cover back into position so that it is aligned with the rear latches and clicks closed. CAUTION: Take care when installing the cover. Do not press or push the lower-front area, as shown below with the red "X." This can damage the scanner's assembly. Place your hand flat against the areas shown with the green check mark, and then gently push to slide the cover back into place. 4 Turn on the scanner. Transporting or moving the Aperio GT 450 If you need to relocate the scanner, contact Leica Biosystems Technical Services. Be aware that moving the scanner yourself may void the hardware warranty. Long term storage If you are not going to use the scanner for a considerable amount of time, shut it down and unplug it. To shut down the scanner, follow the steps in Shut down the scanner (on page 75). If you want to store the scanner, contact Leica Biosystems Technical Services for assistance. For information on disposing of the scanner, see Disposal of the Aperio GT 450 (on page 19). Aperio GT 450 User's Guide, MAN-0391 Revision G 92 Copyright © 2024 Leica Biosystems Imaging, Inc.  5 Maintenance Scanner Maintenance Log Scanner Model Number: Scanner Serial Number: Year Installed: Year of Log: Activity Technician Date Date Date Date Date Date Date Date Date Date Date Date Date Date Daily Maintenance Restart scanner *Clean pusher/puller *Clean slide tray Monthly Maintenance Clean objective and Koehler Clean carousel Clean slide racks Clean fan filter Clean touchscreen Clean scanner cover Yearly Maintenance Schedule annual maintenance visit *Perform visual inspection daily; clean if you observe debris. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc. 93 6 Troubleshooting This chapter contains information and instructions to help you resolve troubleshooting issues with your scanner. For issues with the Aperio GT 450 Scanner Administration Manager, see the Aperio GT 450 IT Manager and Lab Administrator's Guide. WARNING: Do not attempt to perform troubleshooting procedures not shown in this chapter. For additional troubleshooting assistance, contact Leica Biosystems Technical Services. In this section: Personal protective equipment 95 Red blinking lights on carousel 95 How to use the troubleshooting steps 95 Perform a safe restart after an error 96 Error codes and solutions 99 Rack warnings and solutions 115 Symptoms and solutions 123 Aperio GT 450 User's Guide, MAN-0391 Revision G 94 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting Personal protective equipment If you need to access the interior of the scanner while troubleshooting an issue, follow the policies and procedures of your institution, including the use of personal protective equipment (PPE). Red blinking lights on carousel If the lights in front of the carousel are blinking red, the scanner needs attention. If there is an issue with a rack in the rack loading area, the light below that rack position blinks red. See the next section for more details on resolving scanner issues. How to use the troubleshooting steps The following table describes how to find the appropriate troubleshooting section: Type of issue Scanner status Steps to resolve the issue An error message box similar to this example Until the error is resolved: 1 You can tap the to appears on the touchscreen interface: minimize the message box. l You cannot interact with the This enables you to view the carousel. rack status and access the Help resources. l The scanner cannot continue 2 See Error codes and solutions scanning. (on page 99) for steps to resolve the specific error. A rack warning and error message similar to this There is a problem with See Rack warnings and solutions example appears on the touchscreen interface: the rack or one or more (on page 115) for steps to resolve slides in the rack. the specific error. The scanner can continue scanning other racks or slides. Aperio GT 450 User's Guide, MAN-0391 Revision G 95 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting Type of issue Scanner status Steps to resolve the issue No message or warning appears on the touchscreen Varies with the situation. See Symptoms and solutions (on interface, but there is a problem with the scanner. page 123). For example, the scanner does not power up when you turn it on. Perform a safe restart after an error Some procedures in this chapter ask you to restart the scanner. Restarting the scanner initializes the controller and puts the stage and autoloader in their home positions. Before restarting the scanner, you need to ensure there is no slide on the stage. Follow these steps to safely restart the scanner after an error: 1 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. Aperio GT 450 User's Guide, MAN-0391 Revision G 96 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). 2 Check if there is a slide on the stage or partially on the stage. Figure 6-1: Slide on the stage Figure 6-2: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 3 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. 4 If the pusher is extended, return the pusher to the safe (retracted) position. a Align the pusher teeth with the slide stage grooves. Aperio GT 450 User's Guide, MAN-0391 Revision G 97 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting b Slide the stage to the rear of the scanner, as shown. c Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. 5 Close the scanner cover. a Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G 98 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting b Rotate the VPU latch. c Slide the cover until it clicks closed. For detailed steps, see Restart the scanner after interior maintenance (on page 91). 6 Tap Maintenance on the touchscreen interface, then tap Restart Scanner. Wait for the scanner to complete the restart process. Error codes and solutions If an error box appears on the touchscreen, you must resolve the issue before the scanner can continue scanning. This section contains error codes and messages with troubleshooting steps. 1000: Internal error l Cause: An unexpected event occurred in the system that prevents it from continuing operation. Follow these steps: 1 Open the cover and access the interior: a Open the scanner cover. Aperio GT 450 User's Guide, MAN-0391 Revision G 99 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting b Rotate the VPU latch. c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). 2 Take photos of any obstructions. Leica Biosystems Technical Services may request the photos if you need additional assistance after completing the steps below. 3 Check if there is a slide on the stage or partially on the stage. Figure 6-3: Slide on the stage Figure 6-4: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 4 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. Aperio GT 450 User's Guide, MAN-0391 Revision G 100 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 5 If the pusher is extended, return the pusher to the safe (retracted) position. a Align the pusher teeth with the slide stage grooves. b Slide the stage to the rear of the scanner, as shown. c Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. Aperio GT 450 User's Guide, MAN-0391 Revision G 101 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 6 Close the scanner cover: a Pivot the VPU into place. b Rotate the VPU latch. c Slide the cover until it clicks closed. For detailed steps, see Restart the scanner after interior maintenance (on page 91). 7 Tap Restart Scanner on the touchscreen interface, and wait for the scanner to complete the restart process. 8 If the issue persists, call Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G 102 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 1001: Scanner cannot initialize l Cause: The scanner cannot complete the initialization process. Follow these steps: 1 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. c Pivot out the VPU. For step details, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G 103 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 2 Check if there is a slide on the stage or partially on the stage. Figure 6-5: Slide on the stage Figure 6-6: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 3 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. 4 If the pusher is extended, return the pusher to the safe (retracted) position. a Align the pusher teeth with the slide stage grooves. b Slide the stage to the rear of the scanner, as shown. Aperio GT 450 User's Guide, MAN-0391 Revision G 104 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting c Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. 5 Close the scanner cover: a Pivot the VPU into place. b Rotate the VPU latch. c Slide the cover until it clicks closed. For step details, see Restart the scanner after interior maintenance (on page 91). 6 Shut down the scanner using the steps in Shut down the scanner (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G 105 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 7 Turn on the scanner and wait for the initialization process to complete. 8 If the issue persists, call Leica Biosystems Technical Services. 1002: Carousel cannot rotate l Cause: Something is blocking the light curtain. Follow these steps in order until the issue is resolved and the error message closes: 1 Check the rack loading area and pinch points for any unexpected object. Figure 6-7: Rack loading area with light curtain Figure 6-8: Pinch points on either edge of rack loading highlighted area 2 Ensure the racks in the rack loading area are inserted correctly: Figure 6-9: Leica logo facing out and up Figure 6-10: Rack inserted fully into the rack slot Aperio GT 450 User's Guide, MAN-0391 Revision G 106 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 When you insert the rack, ensure the rack position and the rack status light turns light blue (waiting to scan): Figure 6-11: Empty rack slot Figure 6-12: Rack inserted and waiting to scan 4 Ensure all the slides are fully inserted so that they touch the back of the rack. 5 If there are no obstructions and the carousel still does not rotate, restart the scanner. See Perform a safe restart after an error (on page 96). 6 If the issue persists, contact Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G 107 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 1003: Carousel cannot rotate. Carousel pinch point obstructed. l Cause: There is an obstruction at a pinch point. Follow these steps in order until the issue is resolved and the error message closes: 1 Check the rack loading area and pinch points for any unexpected object that may break the light curtain. Figure 6-13: Rack loading area with light curtain Figure 6-14: Pinch points on either edge of rack loading highlighted area 2 If there are no obstructions and the carousel still does not rotate, restart the scanner by following the steps in Perform a safe restart after an error (on page 96). 3 If the issue persists, contact Leica Biosystems Technical Services. 1007: Internal storage full. Cannot send images to DICOM converter. l Cause: If the internal storage is full, the system cannot send the images to the DICOM converter. The Lab Administrator should perform these steps: 1 Ensure the LAN cables are connected at the scanner LAN port and at the Aperio GT 450 SAM server. 2 Do not restart the scanner. If you restart the scanner, the scanned data is lost, and users have to rescan their slides. 3 Check the connectivity from the scanner to the DICOM server, and from the DICOM server to your site’s image storage location. 4 Ensure the DICOM server is running. Restart the DICOM server if necessary. 5 Check if your site’s image storage location is full. 6 Check if there is a permissions or account problem with the account running the DICOM server. 7 If the issue persists, consult with your organization’s IT professionals prior to calling Leica Biosystems Technical Services. When the issue is resolved, if you have not restarted the scanner, the scanner starts transferring the slide images to the DICOM server. Aperio GT 450 User's Guide, MAN-0391 Revision G 108 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 2000: Slide handling error at slide stage, rack, or pusher. l Cause: There is an obstruction at the slide stage, rack, or pusher. Follow these steps in order until the issue is resolved: 1 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). 2 Take photos of the obstruction. Leica Biosystems Technical Services may request the photos if you need additional assistance after completing the steps below. Aperio GT 450 User's Guide, MAN-0391 Revision G 109 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 Check if there is a slide on the stage or partially on the stage. Figure 6-15: Slide on the stage Figure 6-16: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 4 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. 5 Check the slide stage, rack, and pusher area for an obstruction. 6 If possible, carefully remove the slide that is causing the obstruction. WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. 7 Check the slide for preparation issues, such as over-hanging coverslips and label issues. l If there are no obvious preparation issues, re-insert the slide in an available rack after you restart the scanner. l If there are slide preparation issues, correct the slide issues before rescanning. Aperio GT 450 User's Guide, MAN-0391 Revision G 110 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 8 If the pusher is extended, return the pusher to the safe (retracted) position. a Align the pusher teeth with the slide stage grooves. b Slide the stage to the rear of the scanner, as shown. c Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. Aperio GT 450 User's Guide, MAN-0391 Revision G 111 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 9 Close the scanner cover: a Pivot the VPU into place. b Rotate the VPU latch. c Slide the cover until it clicks closed. For detailed steps, see Restart the scanner after interior maintenance (on page 91). 10 Tap Restart Scanner on the touchscreen interface, and wait for the scanner to complete the restart process. 11 If the issue persists, contact Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G 112 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 2001: Slide handling error at rack gripper, lift, or carousel. l Cause: There is an obstruction near the rack gripper, lift, or carousel. Follow these steps until the issue is resolved: 1 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. c Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). 2 Take photos of the obstruction. WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G 113 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 Check if there is a slide on the stage or partially on the stage. Figure 6-17: Slide on the stage Figure 6-18: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 4 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. 5 Check the rack gripper, lift, and carousel area for an obstruction. 6 Close the scanner cover: a Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G 114 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting b Rotate the VPU latch. c Slide the cover until it clicks closed. For detailed steps, see Restart the scanner after interior maintenance (on page 91). 7 Contact Leica Biosystems Technical Services. 9000: Scanner cover is open l The scanner cover is not closed completely. Follow these steps until the issue is resolved: 1 Slide the cover to the closed position, making sure the cover aligns with rear latches and clicks closed: Rack warnings and solutions Rack warnings indicate a problem with the rack or one or more slides in the rack. The scanner can continue scanning when there is a rack warning. CAUTION: If you need to remove a rack before all slides in the rack are scanned, first take note of the rack and slide status. After you remove a rack, the rack’s scan status is no longer available on the Home screen. Aperio GT 450 User's Guide, MAN-0391 Revision G 115 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 1005: Cannot process rack l Cause: There is an issue with a rack that prevents scanning. Follow these steps in order until the issue is resolved: 1 Rotate the rack to the rack loading area. See Rotate a rack to the rack loading area (on page 55). 2 Ensure the rack is inserted correctly: Figure 6-19: Leica logo facing out and up Figure 6-20: Rack inserted fully into the carousel 3 Remove the rack and check the following: l You are using a supported rack. See Slide racks supported (on page 29). l The rack is not damaged or modified. 4 Verify the error only occurs on one rack. l If the error only occurs on one rack, go to step 5. l If the error occurs on multiple racks, contact Leica Biosystems Technical Services. 5 If you verified the rack is a supported and undamaged rack, reinsert it into the carousel for scanning. 6 If the scanner still cannot process the rack, attempt to restart the scanner by following the steps in Perform a safe restart after an error (on page 96). 7 If the issue persists, remove the rack and contact Leica Biosystems Technical Services. 1006: Cannot process one or more slides in rack l Cause: There is an issue with one or more slides in the rack. Follow these steps in order until the issue is resolved: 1 On the touchscreen, tap the rack position that has the error, and tap Rack View to identify which slides have the error. 2 Tap Slide View to view the macro image of the slide and check for an error message. Aperio GT 450 User's Guide, MAN-0391 Revision G 116 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 Go to the section that matches the slide error: l Tilted slide(s) (on page 117) l No barcode (on page 118) l No tissue (on page 118) l No macro focus (on page 119) l Image quality (on page 119) l Image transfer error – pending retry (on page 120) l Aborted (on page 121) 4 If the issue persists after following the relevant procedure, keep the slide available for inspection and contact Leica Biosystems Technical Services. Slide errors and solutions If there is an issue scanning a slide, one of the following error messages appears in Slide View. Follow the steps in order until the issue is resolved. If you follow the steps and the issue persists, contact Leica Biosystems Technical Services. You can temporarily hide some error messages by tapping the x in the top-right corner. This enables you to view the macro image. Tilted slide(s) l Cause: The slide is tilted between two or more slots in the rack and cannot be scanned. Follow these steps in order: 1 Remove the rack from the carousel, and locate the tilted slide: 2 Insert the slide in a new rack for scanning, ensuring it sits level horizontally in one slot. 3 Insert the rack into an empty rack slot for scanning. Aperio GT 450 User's Guide, MAN-0391 Revision G 117 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting No barcode l Cause: The scanner does not detect a barcode on the slide. The scanner produces an image even if there are no barcodes detected. If your site does not require the use of barcodes, you can ignore this error. Your Lab Administrator determines whether the scanner requires the presence of a barcode to complete a scan. Your Lab Administrator or Leica Biosystems representative sets this configuration in Aperio GT 450 SAM. If your site requires the use of barcodes, follow these steps in order until the issue is resolved: 1 Check the slides and rack are inserted correctly: l Slide label facing out and up. l Leica logo facing out and up. l See Load slides in the rack (on page 43). 2 Ensure barcodes meet specifications. See Barcodes supported (on page 29). 3 Ensure barcodes meet minimum quality requirements. See Barcodes (on page 36). 4 If the issue persists, call Leica Biosystems Technical Services. No tissue l Cause: The scanner does not detect tissue on the slide. Follow these steps in order until the issue is resolved: 1 On the touchscreen interface, tap the rack position that has the error. 2 Tap the Slide View icon to view the macro image of the slide. 3 Tap the error message to temporarily hide it. 4 If available on your scanner, scan the slide again using the optional Manual Scan process. (For details, see Use Manual Scan to resolve an image quality issue (on page 67).) If you do not have the Manual Scan feature available, skip to the next step. a Consider placing additional focus points on the tissue area using the Manual Scan feature. b When the scan is finished, do not remove the rack from the scanner. 5 If the issue persists, continue with the following steps. 6 Verify there is tissue on the slide. 7 Take note of the slide number. Aperio GT 450 User's Guide, MAN-0391 Revision G 118 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 8 Rotate and remove the rack to access the slide. 9 Remove the slide from the rack, and then clean the slide. 10 Re-insert the slide into a new rack and scan it again. 11 If the issue persists, check for slide preparation errors. See Chapter 3: Slide preparation. 12 If the issue persists after completing the previous steps, call Leica Biosystems Technical Services. No macro focus l Cause: The scanner’s camera cannot focus on the tissue. Follow these steps in order until the issue is resolved: 1 Check for slide loading issues: l Slide orientation is correct with the specimen-side facing up. See Load slides in the rack (on page 43). l Slide tray is clean. 2 Check the stain quality. 3 Check that the slide and coverslip thickness meet the requirements. See the Aperio GT 450 Specifications. 4 Check for common slide preparation issues and verify: l Coverslip is not missing or overhanging. l Labels are not overhanging or on wrong side. l There is only one label applied to the slide. l Slide is clean. 5 If every slide has the same error, or if the issue persists after following the previous steps, call Leica Biosystems Technical Services. Image quality l Cause: The scanner’s Auto-Image QC feature detected an image quality issue. Follow these steps in order until the issue is resolved: 1 Review the scanned slide image in your viewing software. 2 Check for common slide preparation issues and verify: l Coverslip is not missing or overhanging. l Labels are not overhanging or on wrong side. l There is only one label applied to the slide. l Slide is clean. Aperio GT 450 User's Guide, MAN-0391 Revision G 119 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 Display the slide in Slide View, and do one of the following depending on the configuration of your scanner: l If the optional Manual Scan feature is available on your scanner, tap Manual Scan. For detailed instructions, see Use Manual Scan to resolve an image quality issue (on page 67). When the scan is finished, do not remove the rack from the scanner. l If your scanner does not have the Manual Scan feature available, tap Scan Entire Slide to re-scan the entire slide. (To scan the whole area for a rack of slides, see Scan entire slide for whole rack (on page 63).) 4 When the scan is finished, do not remove the rack from the scanner. 5 Review the newly scanned slide image in your viewing software. If the issue persists, continue with the following steps. 6 Check if other slides are in focus. 7 If all slides are out of focus, clean the objective. See Clean the objective and Koehler (on page 82). 8 If the issue persists after following the previous steps, call Leica Biosystems Technical Services. Image transfer error – pending retry l Cause: The scanner cannot transfer the scanned image to the image storage location. Follow the appropriate steps below: Error appears on some slides Error appears on all slides The system often resolves the issue without The Lab Administrator should: intervention. 1 Ensure the LAN cables are connected to the scanner LAN port and 1 If all slides in the rack are finished to the Aperio GT 450 SAM server. scanning, remove the rack. 2 Do not restart the scanner. If you restart the scanner, the scanned 2 Check for the image in Aperio eSlide data is lost, and users have to rescan their slides. Manager. 3 Check the connectivity from the scanner to the DICOM server, and 3 If necessary, rescan only the slides that from the DICOM server to your site’s image storage location are missing from Aperio eSlide Manager. 4 Ensure the DICOM server is running. Restart the DICOM server if necessary. 5 Check if your site’s image storage location is full. 6 Check if there is a permissions or account problem with the account running the DICOM server. 7 If the issue persists, consult with your organization’s IT professionals prior to calling Leica Biosystems Technical Services. When the issue is resolved, if you have not restarted the scanner, the scanner starts transferring the slide images to the DICOM server. Aperio GT 450 User's Guide, MAN-0391 Revision G 120 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting Aborted l Cause: The scanner cannot scan the slide. Follow these steps in order until the issue is resolved. 1 If the “Aborted” message appears on all slides, go to Aborted message appears on all slides (on page 121). If the message appears on one or some slides, continue to the next step. 2 Check for damage or common slide preparation issues and verify: l Coverslip is not missing or overhanging. l Labels are not overhanging or on wrong side. l There is only one label applied to the slide. l There is only one coverslip applied to the slide. l Slide is clean. 3 Clean the slide. 4 Insert the slide into a different rack and scan it again. 5 If the issue persists after following the previous steps, call Leica Biosystems Technical Services. Aborted message appears on all slides Follow these steps in order until the issue is resolved: 1 Remove any completed slide racks from the carousel. 2 Open the cover and access the interior: a Open the scanner cover. b Rotate the VPU latch. Aperio GT 450 User's Guide, MAN-0391 Revision G 121 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting c Pivot out the VPU. For step details, see Open the scanner cover and access the interior components (on page 75). 3 Check if there is a slide on the stage or partially on the stage. Figure 6-21: Slide on the stage Figure 6-22: Slide partially on the stage CAUTION: Restarting the scanner with a slide on the stage may damage the slide. 4 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. 5 Close the scanner cover: a Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G 122 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting b Rotate the VPU latch. c Slide the cover until it clicks closed. For detailed steps, see Restart the scanner after interior maintenance (on page 91). 6 Shut down the scanner by tapping Maintenance, then tapping Shut Down Scanner. 7 When touchscreen is dark, turn the scanner off using the On/Off switch. 8 Turn the scanner back on using the On/Off switch. 9 Allow the scanner to scan any remaining racks. 10 If the issue persists, call Leica Biosystems Technical Services. Artifact detection messages from the optional Aperio iQC Software Module If you are using the optional Aperio iQC Software Module, messages preceeded by "iQC" appear in the scanner console's Slide View if the Aperio iQC Software Module detects one or more artifacts on the scanned image. Some examples of messages include "iQC: Missing Tissue," "iQC: Air Bubbles," etc. See your Aperio iQC Software Module User's Guide for full details. Symptoms and solutions This section contains troubleshooting information organized by symptom for scanner issues that do not have an error message or code. Scanner does not power on 1 Ensure the scanner is turned on. 2 If you are using an optional uninterruptible power supply (UPS), ensure it is turned on. Aperio GT 450 User's Guide, MAN-0391 Revision G 123 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting 3 Check the connections to wall outlet, and the Ethernet cable that attaches to the back of the scanner. 4 Ensure there is power available where the scanner is plugged in. 5 Verify there is a network connection to the device. 6 Ensure the main scanner cover is fully closed. 7 If the issue persists, call Leica Biosystems Technical Services. Touchscreen does not respond to touch 1 Shut down the scanner by following the steps in Shut down the scanner (on page 75). 2 Turn the scanner on. 3 If the issue persists, contact Leica Biosystems Technical Services with details. Touchscreen is black 1 Shut down the scanner by following the steps in Shut down the scanner (on page 75). 2 Turn the scanner on. 3 If the issue persists, contact Leica Biosystems Technical Services with details. Slides are broken inside the scanner WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. 1 Leica Biosystems Technical Services may request photos when providing assistance. Network connection lost The Aperio GT 450 must be connected to the Aperio GT 450 Scanner Administration Manager (Aperio GT 450 SAM) through your Local Area Network to function. If that connection is lost, you see: Aperio GT 450 User's Guide, MAN-0391 Revision G 124 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting Contact your IT Administrator for assistance. The IT Administrator should do the following: 1 Ensure the LAN cables are connected to the scanner LAN port and to theAperio GT 450 SAM server. 2 In the area provided on the scanner's touchscreen interface, enter the IP address of the Aperio GT 450 SAM server. 3 Verify the network connections are up and working for the Scanner and Aperio GT 450 SAM server. (Consult your organization's IT professionals if needed.) 4 On the server, go to the Services Manager and restart all services. It may take a few minutes for all the services to restart. 5 Try to connect from the scanner again by manually entering the IP address again. 6 If the issue persists, consult with your organization’s IT professionals prior to calling Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G 125 Copyright © 2024 Leica Biosystems Imaging, Inc.  6 Troubleshooting White striping appears on scanned images If your scanned images frequently appear with white striping, this may be caused by certain artifacts introduced by improper tissue processing, such as microscopic contamination, micro water droplets, micro air bubbles, preventing the scanner from obtaining a clear calibration pre-scan. The example below shows an image with thewhite striping effect: The optional Default Calibration Point feature addesses this issue. When the Default Calibration Point is enabled, the scanner checks the quality of the pre-scan during the scan workflow, and if necessary, replaces it with a higher quality default pre-scan that was created specifically for your particular scanner. Your Lab Administrator configures the Default Calibration Point feature in the Aperio GT 450 SAM. See the Aperio GT 450 IT Manager and Lab Administrator's Guide for details. If your scanner is configured with the Default Calibration Point feature, and your scanned images still have the white striping effect, contact Leica Biosystems Technical Services. The Default Calibration Point settings may need adjustments. Aperio GT 450 User's Guide, MAN-0391 Revision G 126 Copyright © 2024 Leica Biosystems Imaging, Inc. Index cover See also scanner cover 2 open and close 75 coverslips 28, 35 20x magnification scanning 47 4 D daily maintenance 77 40x 28 DICOM 28 DICOM converter error 108 A disposal 19 add racks 44 add slides to rack 43 E Auto-Image QC 28 electromagnetic warnings 16 error codes, solutions for 99 B rack warnings 115 barcodes 36 error message 95 barcodes supported 29 Extended Focus 28 blinking lights, red 95 broken slides, recover 124 F fan filter C clean 88 cannot process rack 116 remove 88 cannot rack process error 116 capacity, slide 29 I carousel 24 image quality check, automatic 28 clean 87 image quality review 64 lights 95 image types. supported 28 carousel cannot rotate 106 insert racks 44 clean Koehler 82 insert slides 43 clean objective 82 installation 18 continuous load 28 instrument warnings 17 Aperio GT 450 User's Guide, MAN-0391 Revision G 127 Copyright © 2024 Leica Biosystems Imaging, Inc.  Index internal storage full 108 open cover 75 options L priority 46 orange status 61 labels, requirements 35 overview labels, slides 35 scanner 23 legend 26 touchscreen interface 25 light curtain 24 load racks in scanner 44 P load slides in rack 43 personal protective equipment 95 pinch point obstruction error 108 M power on problem 123 magnification, scan 28, 47 PPE 95 maintenance preparing slides 31 cleaning slide tray 85 priority scan 46 daily 77 pusher, safe position 97, 101, 104, 111 schedule 74 six-month 82 Manual Scan 28 Q messages quality check, image 28 rack issues 95 scanner error 95 slide issues 95 R move scanner 92 rack check status 58 N load in scanner load slides in 44 43 network connection lost 124 unload 56 number of slides 29 warning 61 rack order 60 O rack warning 95 current scan 58 objective racks clean 82 clean 90 location 84 red blinking lights 95 on/off switch 24 remove rack 56 Aperio GT 450 User's Guide, MAN-0391 Revision G 128 Copyright © 2024 Leica Biosystems Imaging, Inc.  Index reports, scan 66 clean slide racks 90 restart scanner clean stage slide tray 85 clean touchscreen 91 after an error 96 daily maintenance 77 slide capacity 29 slide handling error 109, 113 S slide labels requirements 35 safety instructions 19 slide preparation 31 safety light curtain 24 coverslips 35 scan area 65 fix errors 34 scan entire slide labels 35 single slide 60 slide racks whole rack 63 clean 90 scan magnification 28 slide staining requirements 33 scan statistics 66 slide view 60 scan workflow 41 slides, broken 124 scanner slides, load in rack 43 close cover 91 stage slide tray, clean 85 disposal 19 move 92 staining 33 open cover 75 staining requirements 33 restart 77-78 statistics, scan 66 restart after maintenance 91 status legend 26 storage 92 status, rack Scanner Administration Manager 28 descriptions 58 scanner cover status, slide 59 clean 90 storage scanner not powered on 123 scanner 92 scanner safety instructions 19 sensors, light 24 shut down scanner 75 T tissue preparation 33 six-month maintenance touchscreen clean carousel 87 clean fan filter 88 clean 91 clean objective 82 touchscreen interface overview 25 clean scanner cover 90 touchscreen problem 124 Aperio GT 450 User's Guide, MAN-0391 Revision G 129 Copyright © 2024 Leica Biosystems Imaging, Inc.  Index troubleshooting 94 error codes 99 error messages 95 how to use steps 95 rack warnings 115 symptoms 123 touchscreen 124 turn on scanner 24 U UI See touchscreen unload racks 56 unload slides 56 user interface 25, See also touchscreen V VPU close 91 open 75 W warnings rack 61, 115 whole slide scan See scan entire slide workflow, scan 41 Z Z-stack scanning 28, 49 Aperio GT 450 User's Guide, MAN-0391 Revision G 130 Copyright © 2024 Leica Biosystems Imaging, Inc.   Erki Fels Meie 17.12.2024 nr 12.2-10/244 Gregor Saluveer GartnerGrupp OÜ esindajad e-post: [email protected] Raul Tohv Võru Linnavalitsuse esindaja e-post: [email protected] [email protected] Janar Kuus RoadWest OÜ esindaja e-post: [email protected] Kirjaliku menetluse teade Seoses GartnerGrupp OÜ (edaspidi Vaidlustaja) poolt Võru Linnavalitsuse (edaspidi Hankija) riigihankes „Tänavate korrashoiutööd 2025-2030“ (viitenumber 284897) esitatud vaidlustuse nõude ja aluse muutmise ja täiendamisega 16.12.2024 ( riigihangete seaduse (edaspidi RHS) § 191 lg 1 alusel), Riigihangete vaidlustuskomisjon (edaspidi vaidlustuskomisjon): 1) tunnistab kehtetuks oma 16.12.2024 kirjaliku menetluse teate nr 12.2-10/244 menetlusdokumentide esitamise ning vaidlustuskomisjoni otsuse avaldamise tähtaegade osas; 2) määrab vaidlustusmenetluses uued tähtajad seisukohtade esitamiseks järgmiselt: - Hankijale ja kolmandale isikule (RoadWest OÜ) vastuse esitamiseks ainult Vaidlustaja 16.12.2024 menetlusdokumendile - 20.12.2024; - Vaidlustajale täiendavate seisukohtade esitamiseks kogu vaidlustuse osas – 30.12.2024; - Hankijale ja kolmandale isikule (RoadWest OÜ) vastamiseks Vaidlustaja 30.12.2024 menetlusdokumendile – 03.01.2025. RHS § 196 lg 3 ja RHS § 200 lg 1 alusel teatab vaidlustuskomisjon, et otsuse avalikult teatavaks tegemise aeg on 10.01.2025. Otsus edastatakse menetlusosaliste e-posti aadressidele. Palume kõik menetlusdokumendid saata samaaegselt ka teistele menetlusosalistele. Lugupidamisega (allkirjastatud digitaalselt) Taivo Kivistik Riigihangete vaidlustuskomisjoni liige Lisa: Vaidlustuse täiendus Tartu mnt 85 / 10115 Tallinn / 611 3711 / [email protected]