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REFERENCE MATERIAL DATASHEET 1 (4) File number: Dnr 2025/02935 Reference Material Datasheet Version: 1.0 Issue date: 2025-08-01 Designation: RM Food 2025:8 Batch no: 406 Date of production: 2024-06-26 Manufacturer: Swedish Food Agency, Sweden Storage: –18 °C or lower (but not lower than –55 °C) Batch expiry date: 2026-12-31 Manufacturer and contact information Swedish Food Agency Company name Swedish Food Agency (Livsmedelsverket) Department Unit for Microbiology Address P.O. Box 622 Post code SE-75126 City Uppsala Country Sweden Telephone +46 18 17 55 00 E-mail [email protected] Intended use This reference material is designed for internal quality control of analytical work at food microbiology laboratories. After reconstitution, the test material can be used for control of quantitative food microbiology analyses, as well as for direct or indirect quality control of microbiological media. Content Table 1. Microorganisms included in RM Food 2025:8 Microorganism Strain* Penicillium roqueforti SLV-510 Cladosporium cladosporioides SLV-488 Saccharomyces cerevisiae SLV-375 * Internal strain identification number, Swedish Food Agency Quality control The reference material has been tested for homogeneity at the Swedish Food Agency. No statistically relevant difference has been observed between vials.  REFERENCE MATERIAL DATASHEET 2 (4) File number: Dnr 2025/02935 Property values Table 2. Quality control of RM Food 2025:8. The results are from analysis of 10 individual vials, and are valid for a reconstitution volume of 104 ml. All values are expressed in log10 cfu ml-1. Analysis xRM sRM uRM Acceptance limits Method Yeasts 3.12 0.13 0.38 2.35 – 3.88 NMKL 98:2005 (DG18, 7 days at 25 °C) Moulds 3.22 0.06 0.36 2.50 – 3.94 NMKL 98:2005 (DG18, 7 days at 25 °C) xRM: Property value, to be used for start-up control chart. sRM: Standard deviation of the property value, can be used for start-up control chart. uRM: Standard uncertainty of the property value (includes uncertainty contributions from characterisation, homogeneity, transportation and method differences). The lower/upper acceptance limits are calculated as: xRM ± 2 * uRM (expanded uncertainty at a 95 % confidence interval, with k = 2) Commutability The values in Table 2 are calculated with and apply for the respective methods specified. They are in general applicable to other similar methods, but results may differ from those determined during the quality control at the Swedish Food Agency. The standard uncertainties (uRM) of the property values include uncertainty contributions from method differences (sR), which are calculated from historical distributions of results from different methods on PT samples with a similar composition. The standard uncertainties and acceptance limits can thus in most cases be considered applicable for comparable microbiological methods. Traceability Homogeneity, property values, standard deviations and control limits are calculated in accordance with ISO 17034 and ISO Guide 35. All values are metrologically traceable to the respective strains in the Swedish Food Agency’s internal culture collection (Table 1). Preparation of simulated food sample Reconstitute the vial content according to the instructions on the last page. Please note that the final 104 ml corresponds to the undiluted sample to be analysed. The Swedish Food Agency uses peptone water (0.1 %) with NaCl (0.85 %) as diluent. Laboratories are however advised to use the same diluent as they normally use for the analysis of yeasts and moulds. Analyses The analyses should be performed in accordance with the methods used by the individual laboratory. Control charts Instructions for the construction of control charts are available at our website: www.livsmedelsverket.se/RM-micro  REFERENCE MATERIAL DATASHEET 3 (4) File number: Dnr 2025/02935 Approved by Jonas Ilbäck Jonas Ilbäck RM Production Coordinator  REFERENCE MATERIAL DATASHEET 4 (4) File number: Dnr 2025/02935 Sample preparation of freeze-dried cultures in glass vial (RM Food) 1. Twist the flap on the aluminium cap in the 3. Remove the rubber plug. direction of the arrow. 4. Carefully burn the opening of the vial over a gas 2. Remove the aluminium cap. flame. 5. Add 1 ml diluent with a sterile pipette. 9. Transfer the suspension to the bottle containing 6. Let the content dissolve (1-5 minutes). 100 ml diluent. 7. Using a sterile Pasteur pipette, transfer the 10. Repeat steps 8 and 9 two more times with the suspension to a sterile bottle containing 100 ml same Pasteur pipette. room temperature diluent. 11. After thorough intermittent mixing, the 104 ml 8. Add another 1 ml and carefully rinse the walls of sample is ready for analysis. the vial with the Pasteur pipette. 12. Perform the analyses within 60 minutes.* * From experience, for analysis of moulds more accurate results are often obtained if the final 104 ml sample is allowed to rest for 30 minutes (followed by a new mixing) prior to analysis. Saatja: [email protected] Saaja: "Undisclosed recipients:" Teema: New Reference Material - RM Food 2025:8 Kuupäev: 2025-08-01 08:14 Tähelepanu! Tegemist on väljastpoolt asutust saabunud kirjaga. Tundmatu saatja korral palume linke ja faile mitte avada. Dear customer, We’re pleased to inform you that a new RM batch – RM Food 2025:8 – is now available for food analyses on yeast and mould. The new RM Food 2025:8 replaces the current RM Food 2021:8 (which is still valid until 2025-12-31). Orders are made on our web portal: <https://laboratory.livsmedelsverket.se/> https://laboratory.livsmedelsverket.se Please visit our webpage for more information: Swedish: https://www.livsmedelsverket.se/RM-micro <https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.liv smedelsverket.se%2FRM-micro&data=05%7C01%7Cjoan.hansen%40atria.com%7C74461 1b9526d4f1874b408da7f82c290%7Cacb0bb40b7524e5ead12f890ffb273ae%7C0%7C0%7C6 37962496833022018%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2lu MzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NbKeslMhKuGyLPCtl 5vgBKpbyQ0OFWHQNORz3xanIp4%3D&reserved=0> English: https://www.livsmedelsverket.se/en/RM-micro <https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.liv smedelsverket.se%2Fen%2FRM-micro&data=05%7C01%7Cjoan.hansen%40atria.com%7C 744611b9526d4f1874b408da7f82c290%7Cacb0bb40b7524e5ead12f890ffb273ae%7C0%7C 0%7C637962496833022018%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjo iV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=4EIrlGwHUEjf Qpw3FMNA%2BPaMK22VyMI3eKKV5Af2pdM%3D&reserved=0> Best regards, Jonas Ilbäck | Microbiologist, PhD Swedish Food Agency | Unit for Microbiology PO box 622 | SE-75126 Uppsala | Sweden Phone (switchboard): +46 18 17 55 00 <mailto:[email protected]> [email protected] | <https://www.livsmedelsverket.se/en/PT-micro> www.livsmedelsverket.se/en/PT-micro Web portal: <https://laboratory.livsmedelsverket.se/> https://laboratory.livsmedelsverket.se Food that is good for everyone – now and in the future <https://www.livsmedelsverket.se/en/gdpr> The processing of personal data at the Swedish Food Agency